高吞吐量产生原微粒用于细胞外膀生产和治疗输送
Samantha Ali1, Fabiana Mastantuono1, Andrea Orozco-Torres1
1Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL, 32611 United States.
概括
研究人员开发了一种新的微流体平台,用于生产高通量原体微珠. 这种生物未经修改的系统为先进的3D生物制造和组织工程应用提供了可调节的特性.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 微流体学 微流体学
背景情况:
- I型原蛋白对于组织再生至关重要,但很难将其加工成可扩展的,生物忠实的3D材料.
- 现有的高通量方法往往会损害原生原蛋白结构或限制多功能性.
研究的目的:
- 开发一个可扩展的,生物未经修改的系统,用于原体微珠生成.
- 创建一个压力调节的微流体平台,以快速,高吞吐量制造具有控制性质的原体微珠.
主要方法:
- 使用可调压的微流体平台来产生原体微珠.
- 调整了原度 (0.25-3毫克/毫升-1) 和热自组合.
- 通过SEM来表征微珠大小,纤维结构,机械性能和孔隙结构.
主要成果:
- 产生的单分散微珠 (45-1000微米) 的速率高于10,000个珠子min-1 ,保持本地三螺旋结构.
- 达到可调节的刚度 (0.6-2.8 kPa) 和受控的孔隙结构.
- 证明了增强的生物墨水特性,并支持显著的细胞增殖 (21天内增长13倍).
结论:
- 开发的平台能够快速,高吞吐量生成可调节的,生物未经修改的原体微珠.
- 这些微珠作为3D微环境,细胞培养和生物打印的多功能构建块.
- 这项技术推进了组织建模,药物测试和细胞外囊泡生物制造.
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