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长非编码RNA转录在鱼中脑 - 在无氧和再氧化中注释和表达模式

Magdalena Winklhofer^1,2, Göran Erik Nilsson¹, Sjannie Lefevre¹

¹Section for Physiology and Cell Biology, Department of Biosciences, University of Oslo, Oslo, Norway.

|March 10, 2026

概括

长非编码RNAs (lncRNAs) 在缺氧期间在鱼大脑中被确定. 这些lncRNAs在适应性转录基因对无氧和再氧化的反应中显示出潜在的cis-regulatory作用.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
文字转录学 (Transcriptomics) 是一个学科.

背景情况:

长非编码RNAs (lncRNAs) 是细胞过程的关键调节者.
十字鱼对长期无氧 (缺氧) 具有显著的耐受性.
lncRNAs在鱼无氧适应中的作用在很大程度上是未知的.

研究的目的:

在十字鱼中注释 lncRNAs.
调查 lncRNA 在无氧和氧化过程中的转录组变化中的参与.
描述差异表达的 lncRNA 和它们的相互作用伙伴.

主要方法:

以参考为导向的RNA测序数据的组合,来自在normoxia,anoxia和reoxygenation下的鱼大脑.
使用多种计算工具 (CPC2,CNCI,CPAT,FEELnc) 识别 lncRNAs.
对转录和lncRNAs的差异表达分析,以及对cis作用相互作用的预测.

主要成果:

在鱼大脑中发现了6072个lncRNA.
厌氧引发了显著的转录基因反应,其中1321个具有差异表达的lncRNAs.
同表达分析显示,差异表达的 lncRNA 和它们预测的相互作用伙伴之间存在正相关性,这表明它们具有 cis 调节作用.

结论:

这项研究为鱼提供了全面的lncRNA注释.
在无氧和复氧化过程中,lncRNA与附近的基因进行广泛的共同表达.
lncRNAs可能在十字鱼对缺氧的适应性反应中发挥 cis 调节作用.

关键词:

基因表达基因表达基因表达这是下一代RNA测序.动物生理学的比较鱼类鱼类鱼类鱼类的鱼类鱼类.缺氧缺氧是指缺氧的情况.在 mRNA 丰富度上.

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