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拉蛋白与端粒酶RNA结合,支持植物和状细胞端粒酶通路之间的进化关系
Leon Jenner1, Dzmitry Pruchkouski1,2, Barbora Štefanovie2,3
1Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, Brno 61200, Czech Republic.
Nucleic acids research
|March 10, 2026
概括
阿拉比多普西斯塔利亚纳La1 (AtLa1) 蛋白质与端粒酶RNA结合,类似于Tetrahymena LARP7.7. 这表明植物和状动物的RNA生物生成途径得到保护,涉及不同的但相关的蛋白质.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 进化生物学 进化生物学
背景情况:
- 阿拉比多普西斯塔利亚纳La1 (AtLa1) 是一种真正的La蛋白,与没有植物的LARP7蛋白不同.
- 众所周知,LARP7蛋白在模型系统中参与了端粒酶核糖蛋白复杂生物发生.
研究的目的:
- 研究AtLa1与端粒酶RNA (TR) 的相互作用.
- 为了比较植物中的AtLa1与状动物中的LARP7的RNA结合机制.
- 为了描述AtLa1结合伙伴Domino的RNA结合域DUF3223 (DeCL) 的特征.
主要方法:
- 在体外生化测试.
- 微尺度热泳 (MST) 用于分析分子相互作用.
- 对相关蛋白质中保存域的分析.
主要成果:
- AtLa1与端粒酶RNA (TR) 相结合,类似于纤维状LARP7蛋白p65.
- AtLa1增强了TR与端粒酶逆转录酶RNA结合域的结合.
- 在Domino中,DUF3223域被确定为具有TR结合能力的RNA结合域.
结论:
- 植物和状动物端粒酶RNA生物发生可能共享一种保存途径,分别利用真正的La或LARP7类蛋白质.
- DUF3223域在参与RNA处理的植物和状动物蛋白中被进化保存.
- 这些发现突出了植物和状动物之间的RNA生物发生途径中保存的进化关系.
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