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免疫细胞在3D迁移中使用间歇性的整体介导的引力
Tina Czerwinski1, Lars Bischof1, David Böhringer1
1Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen 91058, Germany.
概括
免疫细胞,就像自然杀手 (NK) 细胞一样,可以切换迁移模式以在密集的组织中导航. 这种适应能力有助于它们在需要时使用收缩力来克服物理障碍.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 生物物理学的生物物理.
背景情况:
- 免疫细胞通过组织迁移到目标,通常使用氨体运动.
- 免疫细胞在迁移期间在密集矩阵中克服硬质障碍的机制尚未完全理解.
- 氨基体迁移的特点是速度,但可能不足以导航复杂的组织架构.
研究的目的:
- 研究免疫细胞如何在密集的纤维组织中适应其迁移策略.
- 阐明细胞收缩性和粘附性在免疫细胞透过程中克服硬质障碍的作用.
- 确定细胞机制,使免疫细胞通过具有挑战性的微环境进行导航.
主要方法:
- 使用的自然杀手细胞 (NK92) 和初级免疫细胞 (B,T,NK细胞,中性粒细胞,单细胞).
- 研究了纤维人胎膜 (HAM) 组织中的细胞迁移,并重建了3D原网络.
- 采用时隔共聚焦反射显微镜,同时测量迁移速度,持久性和细胞收缩性.
主要成果:
- 在密集的HAM组织中,NK92细胞从阿米波体转换为收缩的,类似于介质细胞的迁移模式.
- 免疫细胞在迁移爆发期间会产生由动态肌肉素驱动的,以整合素为媒介的收缩力 (高达100nN).
- 这种收缩性行为与细胞被困在狭窄的矩阵毛孔中相关,在多种免疫细胞类型中观察到.
结论:
- 免疫细胞在迁移模式中表现出显著的可塑性,适应组织密度和物理约束.
- 密集矩阵中的固体阻碍触发了整合素介导粘附和细胞收缩性的快速调节.
- 这种适应性迁移策略对于有效的免疫细胞透和在各种免疫细胞亚型中的功能至关重要.
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