相关实验视频
Updated: Mar 12, 2026

07:23
Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
1.4K
在例行实验室环境中,从门诊患者同时采集的血和血清样本中比较葡萄糖度
Robert Müller1,2, Diethard Müller1, Dietmar Plonné1
1MVZ Labor Ravensburg SE and Co., eGbR, Limbach Gruppe, Ravensburg, Germany.
PloS one
|March 10, 2026
概括
在德国门诊诊所的血清葡萄糖测量显示,与血葡萄糖相比,诊断差异很小,这表明类似的糖尿病查有效性. 然而,这两种样本类型都不能完全防止体外糖解,这使得它们对于最终诊断来说不够理想.
科学领域:
- 临床化学 临床化学
- 糖尿病学 糖尿病学
- 实验室医学 实验室医学
背景情况:
- 德国的指导方针建议静脉血用于测量葡萄糖.
- 血清是德国门诊诊所使用的主要样本类型.
- 血清和血葡萄糖之间的差异可能会影响糖尿病的诊断.
研究的目的:
- 在同时采集的血清和血样本中比较葡萄糖度.
- 根据样本类型评估糖尿病的诊断结果差异.
- 在德国的常规门诊环境中评估葡萄糖测量.
主要方法:
- 分析了来自门诊患者的27,864对血清和化 (NaF) 血样本.
- 血清样本在凝血后在现场进行离心;NaF血样本被运输未经离心.
- 葡萄糖水平使用五分位数和应急表中的中位差异进行了比较,以达成分类协议.
主要成果:
- 在100 mg/dL (0.54-1.13 mg/dL) 以上,葡萄糖的差异是最小的.
- 在100 mg/dL以下,差异随着度的下降而增加 (-1.69至-11.05 mg/dL).
- 糖尿病分类的一致性很高 (97.8%在126 mg/dL的截止值),使用NaF血的诊断仅增加了0.3%.
结论:
- 在现场用分离剂凝离心血清显示,在常规门诊环境中,对于糖尿病查而言,与未离心的NaF血清相比,没有临床相关的缺点.
- 无论是血清还是非酸性NaF血,都不能完全抑制体外糖解.
- 这两种样本类型对于确定的糖尿病诊断目的都是不理想的.
相关概念视频
Hypoglycemia and Glucagon
1.1K
Without prolonged fasting, healthy individuals maintain blood glucose levels above 3.5 mM due to a well-adapted neuroendocrine counterregulatory system that effectively prevents acute hypoglycemia, a potentially life-threatening condition. The primary clinical scenarios for hypoglycemia encompass diabetes treatment, inappropriate production of endogenous insulin or insulin-like substances by tumors, and the use of glucose-lowering agents in non-diabetic individuals. Notably, hypoglycemia in the...
1.1K
Diabetes: Symptoms, Diagnosis, and Complications
2.6K
For most patients, experiencing several weeks of polyuria, polydipsia, fatigue, and significant weight loss may indicate the presence of diabetes. Furthermore, adults displaying the phenotypic appearance of type 2 diabetes (particularly those who are obese and not initially insulin-requiring), may have islet cell autoantibodies, suggesting autoimmune-mediated β cell destruction and a diagnosis of latent autoimmune diabetes of adults (LADA). The categorization of glucose homeostasis is...
2.6K
Glucose Homeostasis: Regulation of Blood Glucose
4.9K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
4.9K
Hormones Regulating Blood Glucose
7.9K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
7.9K

