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在现场检测空气传播的食物传播病原体,使用现场部署的重组酶聚合酶放大和CRISPR/Cas12a裂变活性测定

Yeonwoo Jeong¹, Jina Lee², Sangsoo Choi³

¹Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.

Biosensors & bioelectronics

|March 10, 2026

概括

一个新的诊断平台使用复合酶聚合酶放大和CRISPR/Cas12a裂变活性 (RCCVA测定) 快速检测空气中的食物传播细菌. 这项技术通过在现场快速识别病原体来加强食品安全监测.

科学领域:

食品安全和微生物学
生物技术和诊断技术的发展
环境监测环境监测环境监测

背景情况:

越来越多的单人家庭推动了对餐具的需求,需要大规模的食品生产和复杂的供应链.
全球变暖和复杂的供应链增加了对空气中的食物传播细菌污染的易感性.
传统的空气传播细菌检测方法缓慢,劳动密集,不适合快速的现场监测.

研究的目的:

开发一个可在现场部署的诊断平台,用于快速和灵敏地识别空气中的食物传播细菌.
解决实时食品卫生监测常规方法的局限性.
通过早期发现病原体,提高公共卫生和食品安全.

主要方法:

开发一个诊断平台,将复合酶聚合酶放大与CRISPR/Cas12a裂变活性 (RCCVA试验) 结合起来.
使用自主开发的静电空气采样器收集空气中的细菌.
使用便携式异热放大装置进行分析.

主要成果:

在RCCVA试验中,成功检测出四种主要的食源性病原体:金黄色葡萄球菌,沙门氏菌,Listeria monocytogenes和谷菌.
对目标病原体分别达到274.9,4.5,9.5和28.5 CFU/mL的检测极限.
能够在约1小时内 (分析阶段) 在不到45分钟的时间内在现场检测空气传播病原体.

结论:

开发的RCCVA测试平台提供了快速,灵敏和可在现场部署的空气中传播的食物传播细菌的识别.
这项技术具有在食品加工环境中实时监控的巨大潜力.
通过早期检测和控制空气传播病原体,有助于改善公共卫生和食品安全.

关键词:

空气采样采样空气采样空气中的细菌检测检测.可在现场部署的探测系统.食品卫生食品卫生食物传播细菌是通过食物传播的.基于RPACRISPR/Cas 12a的检测方式

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