共享自取样器并行LC-MS/MS平台用于复杂矩阵中的高通量多分析物量化
Shanshan Cai1, Qisheng Zhong2, Jiaqi Liu2
1School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Journal of chromatography. A
|March 10, 2026
概括
一种新的并行液态色谱-并联质谱 (LC-MS/MS) 策略使用共享硬件来分析复杂样品中的多种分析物. 这种方法提高了分析吞吐量和效率,用于多分析量化.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 环境科学 环境科学
背景情况:
- 在复杂矩阵中同时量化各种分析物对单个LC-MS方法具有挑战性.
- 现有的方法经常面临移动相兼容性和死体积的限制,特别是在2D-LC设置中.
- 高通量分析对于现代分析需求至关重要.
研究的目的:
- 开发一个共享自取样器并行的LC-MS/MS策略,以实现高效的多分析物量化.
- 克服传统LC-MS和2D-LC方法的局限性.
- 在复杂的矩阵应用中展示战略的灵活性和性能.
主要方法:
- 开发了一个并行LC-MS/MS系统,其中有两个独立的LC路径共享一个自动采样器和MS检测器.
- 在单个分析周期内实施了两种不同的染色学方法的顺序执行.
- 利用选择性的多重反应监测 (MRM) 采集和数据级信号集成,而不需要跨维的合.
主要成果:
- 在28分钟内成功量化了人体血清中的394种新出现的污染物,使用酸/双方法配置.
- 在使用HILIC/RP列的单个20分钟运行中,在广泛的日志P范围内同时确定了27种低血糖剂.
- 在两种应用中都表现出良好的线性,低量化极限 (对90%以上的分析物<0.20 ng/mL) 和高精度 (RSD <10%).
结论:
- 共享自动采样器并行LC-MS/MS战略为高吞吐量量化提供了一个灵活和高效的平台.
- 这种方法可以提高分析吞吐量,而不需要额外的MS或自动采样器硬件.
- 该策略有效地解决了在复杂矩阵中分析化学多样性分析物的挑战.
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