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相关概念视频

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Updated: Mar 12, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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共享自取样器并行LC-MS/MS平台用于复杂矩阵中的高通量多分析物量化.

Shanshan Cai1, Qisheng Zhong2, Jiaqi Liu2

  • 1School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.

Journal of chromatography. A
|March 10, 2026
PubMed
概括
此摘要是机器生成的。

一种新的并行液态色谱-并联质谱 (LC-MS/MS) 策略使用共享硬件来分析复杂样品中的多种分析物. 这种方法提高了分析吞吐量和效率,用于多分析量化.

关键词:
复杂矩阵的复杂矩阵高通量分析的分析.多分析剂量化定量化平行色谱学 平行色谱学分享自动采样仪LCMS/MS

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科学领域:

  • 分析化学 分析化学
  • 生物化学 生化学
  • 环境科学 环境科学

背景情况:

  • 在复杂矩阵中同时量化各种分析物对单个LC-MS方法具有挑战性.
  • 现有的方法经常面临移动相兼容性和死体积的限制,特别是在2D-LC设置中.
  • 高通量分析对于现代分析需求至关重要.

研究的目的:

  • 开发一个共享自取样器并行的LC-MS/MS策略,以实现高效的多分析物量化.
  • 克服传统LC-MS和2D-LC方法的局限性.
  • 在复杂的矩阵应用中展示战略的灵活性和性能.

主要方法:

  • 开发了一个并行LC-MS/MS系统,其中有两个独立的LC路径共享一个自动采样器和MS检测器.
  • 在单个分析周期内实施了两种不同的染色学方法的顺序执行.
  • 利用选择性的多重反应监测 (MRM) 采集和数据级信号集成,而不需要跨维的合.

主要成果:

  • 在28分钟内成功量化了人体血清中的394种新出现的污染物,使用酸/双方法配置.
  • 在使用HILIC/RP列的单个20分钟运行中,在广泛的日志P范围内同时确定了27种低血糖剂.
  • 在两种应用中都表现出良好的线性,低量化极限 (对90%以上的分析物<0.20 ng/mL) 和高精度 (RSD <10%).

结论:

  • 共享自动采样器并行LC-MS/MS战略为高吞吐量量化提供了一个灵活和高效的平台.
  • 这种方法可以提高分析吞吐量,而不需要额外的MS或自动采样器硬件.
  • 该策略有效地解决了在复杂矩阵中分析化学多样性分析物的挑战.