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Updated: Mar 12, 2026

CD Spectroscopy to Study DNA-Protein Interactions

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MdMYB123及其截断变体的功能分歧调节了果中的Cd吸收和排毒

Litong Zheng¹, Yangyang Yuan¹, Xun Qi¹

¹State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.

Journal of hazardous materials

|March 10, 2026

概括

果转录因子MdMYB123通过激活保护基因来增强 (Cd) 耐受性. 它的截断形式,mdmyb123,增加了灵敏度,突出了MdMYB123.

科学领域:

植物分子生物学植物分子生物学
环境毒理学环境毒理学
农业科学农业科学

背景情况:

农业土壤中的 (Cd) 污染对作物生产率和食品安全构成风险.
了解植物对重金属压力的反应对于开发更安全的作物至关重要.

研究的目的:

调查果转录因子MdMYB123及其变体mdmyb123在耐受性中的作用.
阐明了MdMYB123介导的排毒的基础分子机制.

主要方法:

在暴露下进行基因表达分析.
对MdMYB123和mdmyb123在过度表达的果线,酵母和Arabidopsis中的功能分析.
转录组测序以识别下游目标.
分子测定包括酵母一混合,记者测定和ChIP-qPCR.

主要成果:

过度表达MdMYB123增强了耐受性,减少了Cd积累和氧化应激.
mdmyb123过度表达增加了敏感性和氧化损伤.
MdMYB123和mdmyb123与MdALMT1和MdtDT的促进体结合,但只有MdMYB123可以激活转录.
过度表达MdALMT1和MdtDT导致了对的耐受性.

结论:

通过激活真空封存并通过下游目标MdALMT1和MdtDT限制吸收,MdMYB123赋予了耐受性.
MdMYB123是提高果品种中安全性的关键调节剂.
准MdMYB123为培育无安全的果品种提供了潜力.

关键词:

果果果果果果果是什么意思在Cd的耐受性.转录法规转录法规

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