通过SI可追溯的同位素稀释质谱法描述和开发一种介质素-6参考材料
Ru Yan1, Lihua Wang2, Hongmei Ji2
1School of Life Sciences, Nanjing University, Nanjing 210023, China; Nanjing Institute of Metrological Supervision and Testing, Nanjing 210049, China.
概括
开发并描述了一种新的重组型IN-6 (IL-6) 参考材料. 这种稳定的IL-6材料适用于验证和校准诊断试验,确保可靠的炎症测量.
科学领域:
- 生物化学和分子生物学
- 免疫学 免疫学 免疫学
- 分析化学 分析化学
背景情况:
- 介乐-6 (IL-6) 是一种关键的细胞因子,涉及炎症,免疫反应和代谢功能.
- 准确量化IL-6对于临床诊断和研究至关重要.
- 标准化的参考材料对于测试验证和校准至关重要.
研究的目的:
- 准备和表征一种重组的IN-6 (IL-6) 参考材料 (RM).
- 使用SI可追溯方法确定IL-6质量度的认证值.
- 确保RM适用于验证和校准IL-6检测的适用性.
主要方法:
- 蛋白质组技术用于对重组IL-6的表征.
- 使用SI可追溯的方法,包括氨基酸分析同位素稀释质谱法 (AAA-IDMS) 和基于的同位素稀释质谱法 (-IDMS),用于量化.
- 进行了同质性和稳定性研究,以评估材料的均性和保质期.
- 使用AAA-IDMS进行了九个实验室的合作研究,以赋予认证值.
主要成果:
- 通过AAA-IDMS确定IL-6的质量度为 (79.46 ± 1.80) μg/g,通过-IDMS确定 (78.26 ± 1.94) μg/g,显示出高的一致性.
- 同质性评估证实了RM不同单位的统一性.
- 稳定性研究表明,当RM存储在-80°C时,它至少保持12个月的稳定性.
- 经过认证的IL-6质量度为 (80.09 ± 2.93) μg/g (k=2),包括所有不确定性贡献.
结论:
- 现在可以获得具有良好特征的复合IL-6参考材料.
- RM为生物样本中IL-6的准确测量提供了可靠的标准.
- 这种参考材料将提高临床诊断中IL-6检测的质量和可比性.
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