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在CRISPR-Cas9诱导的人类细胞双链断裂后,针对DNA甲基化变化的原生长读测序

Yingzi Zhang^1,2, Mengge Wang^1,2, Chongwei Bi¹

¹Bioscience Program, Biomedical Sciences Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.

BMC research notes

|March 11, 2026

概括

CRISPR-Cas9 DNA 断裂可以改变局部表观遗传标记,影响基因组稳定性. 现在可以详细研究5mC和5hmC在不同位置的变化的数据集,以便进一步研究.

科学领域:

基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

CRISPR-Cas9对于基因组工程至关重要,它可以创建DNA双链断裂 (DSB).
这些DSB对局部表观遗传信息的长期影响尚不清楚.
在DSB附近的表观遗传维护对基因组稳定性至关重要.

研究的目的:

提供与CRISPR-Cas9诱导的DSB相关的原始和加工数据集的结构化描述.
为了使与DSB相关的表观遗传不稳定性进行重新分析和方法比较.
调查DSB对DNA甲基化 (5mC) 和基甲基化 (5hmC) 在各种基因组环境中的影响.

主要方法:

使用Cas9辅助的向纳米孔测序 (CTS).
产生了5mC和5hmC的基本分辨率测量结果.
数据包括对齐的BAM文件和针对目标和控制条件的每CpG甲基化调用.

主要成果:

已经证明,CRISPR-Cas9诱导的DSBs会破坏局部表观遗传维护.
在印记位置,异色区域和与癌症相关的表皮转移区域观察到表观遗传变化.
数据集涵盖多个细胞系统和基因组背景,包括人类胚胎干细胞 (hESC) 和RKO细胞.

结论:

提供的数据集是研究与DSB相关的表观遗传不稳定性的宝贵资源.
这些数据有助于对长期阅读的甲基化分析工作流程进行比较.
进一步研究基因组工程对表观遗传调节的长期影响是可以的.

关键词:

克里斯普尔-Cas9是什么意思结肠直肠癌的细胞癌.通过DNA甲基化.双链断裂是双链断裂的原因之一.表观遗传不稳定性表观遗传不稳定性基因组印记是指基因组的印记.人类胚胎干细胞人类胚胎干细胞长读序列的测序方式纳米孔测序的测序

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