来自金奇的冷活性β-银酸酶Latilactobacillus sakei J15:酶特性和益生菌潜力
Ruya Tan1, Enze Zhang1, Yaxuan Li1
1School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023 Zhejiang, China.
Journal of AOAC International
|March 11, 2026
概括
一种金分离物Latilactobacillus sakei J15显示出有希望的益生菌潜力,在模拟消化中具有高的酶活性和存活率. 这种菌株适用于低温乳制品发酵.
科学领域:
- 食品微生物学 食品微生物学
- 益生菌研究 益生菌研究
- 乳酸细菌 乳酸细菌 乳酸细菌
背景情况:
- 拉提拉克托巴西勒斯萨基 (Lb. 在发酵食品中发现,适应低温.
- 这项研究的重点是Lb. 萨基J15,从金中分离出来.
研究的目的:
- 研究Lb.的益生菌特性. 萨基 J15.15 年份
- 评估其作为低温发酵起始培养的潜力.
主要方法:
- 在不同温度,盐度和碳来源下评估成长.
- 在模拟的胃肠道条件下 (酸性,胆盐,消化) 评估的生存率.
- 测量了抗氧化活性,自身聚合,溶酶耐受性和血液溶解活性.
主要成果:
- 一个英. 在15°C (789.31 mU/mg) 时,Sakei J15表现出高的β-银酸酶活性.
- 在酸性 (91.5%) 和胆盐 (66.0%) 条件下表现出极好的生存率.
- 对模拟的胃 (63.0%) 和肠道 (47.0%) 消化表现出强烈的耐受性.
- 显示显著的抗氧化活性 (DPPH 43.0%,ABTS 71.9%) 和自动聚合 (96.1%).
- 有效地利用FOS和GOS,具有高的溶酶耐受性 (93.7%) 并且没有溶血活性.
结论:
- 一个英. 萨基J15具有显著的益生菌潜力.
- 它是低温乳制品发酵中初始培养的有希望的候选者.
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