Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

603
Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
603
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

706
Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
706
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

862
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
862
Pathophysiology of Cardiac Performance01:29

Pathophysiology of Cardiac Performance

1.8K
Typical heart performance is influenced by heart rate, rhythm, myocardial contraction, and metabolism or blood flow. The cardiac muscle exhibits distinct electrophysiological features, including pacemaker activity and calcium channel control, which play a vital role in the heart's response to various drugs. The autonomic nervous system, comprising the sympathetic and parasympathetic branches, regulates heart rate. Sympathetic activation increases heart rate, while parasympathetic activation...
1.8K
Mechanism of Cardiac Arrhythmias01:28

Mechanism of Cardiac Arrhythmias

2.5K
Arrhythmias are irregular heart rhythms occurring when the heart's electrical impulses become abnormal. These disturbances can lead to various symptoms, depending on their severity and the underlying cause. Some common factors contributing to arrhythmias include hypoxia, ischemia, electrolyte imbalances, excessive catecholamine exposure, drug toxicity, and muscle overstretching. Arrhythmias can be classified into two main types based on the rate and site of origin of abnormal heart rhythms.
2.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.8K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Metabolic crisis and TRPM4 activation cause QT prolongation in TANGO2 deficiency disorder.

Cardiovascular research·2026
Same author

Genetic ablation of neuronal mitochondrial calcium uptake impedes Alzheimer's disease progression.

The EMBO journal·2026
Same author

MICU proteins facilitate calcium-dependent mitochondrial metabolon formation to regulate cellular energetics independently of MCU.

Nature metabolism·2026
Same author

Dynamic-Structure Redesign of Calmodulin Reveals Mechanistic Constraints on Ryr2 Regulation.

bioRxiv : the preprint server for biology·2026
Same author

YAP1 and QSER1 are key modulators of embryonic signaling pathways in the mammalian epiblast.

EMBO reports·2026
Same author

Structural Evaluation of <i>RYR2</i>-CPVT Missense Variants and Continuous Bayesian Estimates of Their Penetrance.

Circulation. Genomic and precision medicine·2026
Same journal

Editors and Editorial Board.

Circulation research·2026
Same journal

Cannabis Is Associated With Potentially Proatherogenic Inflammation in Youth.

Circulation research·2026
Same journal

WTAP Deficiency Promotes Podocyte PANoptosis and FSGS via SPP1 Signaling.

Circulation research·2026
Same journal

B1a Cell-IgM Axis Protects Against Hypertension by Blunting IFNγ+CD4+T Cells.

Circulation research·2026
Same journal

SBK2 Links Cardiac Stress Signaling to Mitochondrial Proteostasis.

Circulation research·2026
Same journal

Myeloid Piezo1 as a Brake on Efferocytosis and Cardiac Repair in the Infarcted Heart.

Circulation research·2026
查看所有相关文章

相关实验视频

Updated: Mar 12, 2026

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
11:26

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

Published on: February 13, 2019

9.3K

简短的SCN5A转录产生NaV1.5片段,影响心脏新陈代谢.

Nathan H Witmer1,2, Jasmyn M Hoeger1,3, Jared M McLendon1,4

  • 1Department of Internal Medicine, Fraternal Order of Eagles Diabetes Research Center, Abboud Cardiovascular Research Center, Carver College of Medicine, University of Iowa. (N.H.W., J.M.H., J.M.M., C.S.S., J.-Y.Y., B.L.L., R.L.B.).

Circulation research
|March 11, 2026
PubMed
概括
此摘要是机器生成的。

在SCN5A中新发现的一种替代多化信号产生了一个短的转录. 该转录编码了一种线粒体蛋白 (NaV1.5-NT),该蛋白增强心肌细胞代谢,并可能影响心力衰竭.

关键词:
行动潜力 行动潜力心肌病,扩张心肌病,扩张心肌病.阻器,可以植入的.线粒体中的线粒体.的道是一种通道.

更多相关视频

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
11:33

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

Published on: March 12, 2013

13.8K
Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk
05:15

Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Published on: July 27, 2022

2.2K

相关实验视频

Last Updated: Mar 12, 2026

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
11:26

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

Published on: February 13, 2019

9.3K
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
11:33

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

Published on: March 12, 2013

13.8K
Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk
05:15

Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Published on: July 27, 2022

2.2K

科学领域:

  • 心血管生物学 心血管生物学
  • 分子心脏病学分子心脏病学
  • 线粒体的新陈代谢

背景情况:

  • SCN5A编码心脏NaV1.5通道,对于动作潜能和心肌细胞代谢至关重要.
  • SCN5A变异与心力衰竭和心律失常有关.
  • 调节SCN5A表达及其代谢作用的机制尚未完全理解.

研究的目的:

  • 为了确定SCN5A.新的监管特征.
  • 调查SCN5A中保存的替代多化 (APA) 信号的功能作用.
  • 探索一种新型SCN5A衍生蛋白对心肌细胞代谢的影响.

主要方法:

  • 生物信息分析用于识别SCN5A.A中的APA信号.
  • 产生具有人类APA信号的模拟小鼠.
  • 使用西式斑点,细胞分离和显微镜对截断的SCN5A蛋白质异型 (NaV1.5-NT) 的表征.
  • 评估心肌细胞和小鼠心中的线粒体功能和代谢物.

主要成果:

  • 在SCN5A外因子2下游的一个保存的APA信号产生了一个编码为NaV1.5-NT.的短转录 (SCN5A-short).
  • NaV1.5-NT局限于线粒体矩阵,并增强线粒体呼吸,ATP生产和复杂I活性.
  • NaV1.5-NT表达改变心脏代谢组,表明脂肪酸氧化增加.
  • 在失败的人类心脏中观察到减少的SCN5A短表达.

结论:

  • 替代性多基化SCN5A产生一个针对线粒体的 (NaV1.5-NT) 支持心肌细胞代谢.
  • 这一途径揭示了新的SCN5A-线粒体交叉声,这对心力衰竭和心律失常有影响.
  • 需要进一步的研究来阐明NaV1.5-NT功能的精确分子机制.