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基于等离子体Vis-NIR光谱模式识别的隐性乙型肝炎病毒感染的快速和无试剂查

Linbin Huang¹, Xiaoting Huang², Jingjing Xia¹

¹Guangxi Medical University Cancer Hospital, Nanning, China.

Frontiers in bioengineering and biotechnology

|March 11, 2026

概括

隐藏的乙型肝炎病毒感染 (OBI) 可以使用等离子体可见近红外 (Vis-NIR) 光谱检测. 这种快速,低成本的方法可以准确地将OBI与健康对照区分开来,有助于早期诊断.

科学领域:

生物医学光谱学生物医学光谱学
肝病学肝病学是一种肝病学.
传染性疾病传染性疾病

背景情况:

隐性乙型肝炎病毒感染 (OBI) 的特征是无法检测到的乙型肝炎表面抗原 (HBsAg),但存在HBV DNA.
由于HBV DNA测试的高成本和复杂性,OBI往往被诊断不足.
等离子体可见近红外 (Vis-NIR) 光谱学为OBI检测提供了一个潜在的替代方案.

研究的目的:

开发和验证使用Vis-NIR光谱的歧视性分析模型,以区分OBI与健康血.
评估OBI查的基于光谱学模型的准确性和稳定性.
探索Vis-NIR光谱的潜力,以开发低成本的OBI检测方法.

主要方法:

从自愿献血者收集了444个血样本 (204个OBI,240个对照).
测量了等离子体的Vis-NIR光谱和应用的预处理技术 (SNV,NDF).
使用了部分最小方位差异分析 (PLS-DA) 和k-近邻 (kNN) 分类器,具有最佳波长选择 (SDPC-WSP).

主要成果:

成功建立了用于OBI检测的光谱歧视模型.
在独立验证集中,SDPC-WSP-kNN模型实现了96.6%的灵敏度,100%的特异性和98.7%的准确性.
一个灰色判断区进一步改善了检测,达到100%的灵敏度和特异性,恢复率为96.8%.

结论:

视NIR光谱图案识别准确地将OBI与正常等离子体区分开来.
这种无试剂,快速,简单的方法适用于大规模,低成本的OBI查.
从这种技术中获得的短波长模型可以为专门的OBI检测设备的开发提供信息.

关键词:

血液查血液查多个波长的多个波长.诺里斯衍生品过器隐藏的乙型肝炎病毒感染.部分最小平方差异分析.分离度优先组合分离度优先组合一步一步的逐步淘汰.可见近红外频谱的模式识别.

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