通过S-化在Monascus spp中的TCA循环的代谢调节
Zitong Meng1,2,3,4, Yingao Tan1,5, Yali Duan1,5
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China.
Synthetic and systems biotechnology
|March 11, 2026
概括
蛋白质S-基化通过抑制三碳酸 (TCA) 循环来调节莫纳斯克的新陈代谢. 这个过程影响了二次代谢物生产,为增强的Monascus应用提供了洞察力.
科学领域:
- 微生物学和生物化学
- 代谢学和蛋白质学
背景情况:
- *Monascus物种对于生产二次代谢物 (SMs) 很有价值,如Monacolin K和Monascus颜料.
- * 蒙纳斯克的新陈代谢调节受包括基因表达和S-化在内的因素的影响.
- * S-化在Monascus代谢途径中的确切作用尚不清楚.
研究的目的:
- * 调查S-化在Monascus代谢途径中的调节作用和机制.
- * 为了确定Monascus purpureus中的S-化蛋白及其功能分布.
- *阐明S-化对二次代谢物生物合成的影响.
主要方法:
- * 在Monascus purpureus HJ11上使用了定量S-nitrosoproteomics.
- *已识别和量化S-化蛋白质.
- * 进行了相关性分析和体外酶体实验.
主要成果:
- *确定了352种S-化蛋白,占总蛋白质组的12%.
- *S-化蛋白主要参与糖解,TCA循环,氨基酸和脂肪酸代谢.
- * 87%的TCA循环酶是S-基化,这表明抑制了TCA循环.
结论:
- * 蛋白质S-化是Monascus物种中一个关键的代谢调节剂.
- *源调节的S-化抑制TCA循环,影响细胞生长和代谢物产生.
- * 结果为提高莫纳斯科高价值代谢物生产提供了洞察力.
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