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综合性研究显示NR1D1通过缺氧诱导因子-1/氨轴介导Hedyotis diffusa的抗纤维化

Si-Wei Xia¹, Huan Liu¹, Kai-Yao Yang¹

¹Jiangsu Key Laboratory for Pharmacology and Safety Research of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China.

World journal of gastroenterology

|March 11, 2026

概括

扩散 (HD) 通过调节昼夜节律,尿素代谢和缺氧诱导因子-1 (HIF-1) 信号传递,有效地逆转肝纤维化 (HF). 该研究确定NR1D1为关键标,为肝脏疾病提供新的治疗途径.

科学领域:

药理学和中国传统医学
肝病学和肝脏疾病研究研究
分子生物学和信号通路

背景情况:

肝纤维化 (HF) 逆转对于慢性肝病至关重要,但有效的治疗方法有限.
传统中国医学 (TCM) 认为Hedyotis diffusa (HD) 具有抗纤维素的特性,但其机制尚不清楚.
需要对HD的分子路径进行系统的阐明,以验证其治疗潜力.

研究的目的:

调查Hedyotis diffusa (HD) 对肝纤维化 (HF) 的疗效.
探索HD抗纤维效应的潜在分子机制和调节途径.
为了确定HF治疗的潜在治疗点.

主要方法:

在HD注射 (HDI) 的生物活性成分使用液体染色体质谱法进行鉴定.
网络药理学预测了信号通路,并建立了一个HF小鼠模型来验证疗效.
基因表达综合 (GEO) 数据挖掘,肝蛋白学和向基因干扰阐明了抗纤维菌机制.

主要成果:

网络药理学表明,HDI调节昼夜节律,尿素代谢和缺氧诱导因子-1 (HIF-1) 信号传递.
GEO数据和蛋白质组学证实了HF中的途径失调,HDI恢复了昼夜调节器NR1D1.
确定NR1D1是HIF-1信号传导,尿素循环和氨代谢的上游调节器,对LX2细胞激活和线粒体功能有影响.

结论:

扩散性肝炎 (HD) 在治疗肝纤维化 (HF) 中显示出显著的疗效.
通过对昼夜节律基因的影响调节HIF-1和尿素循环途径,HD功能.
NR1D1成为HD抗纤维作用的关键分子标.

关键词:

氨的代谢氨的代谢黑底 (Hedyotis diffusa) 是一个扩散的植物.肝脏纤维化肝脏纤维化缺氧诱导因子-1的诱导因子-1 这就是NR1D1的特点.尿素循环的尿素周期

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