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Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Updated: May 7, 2026

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

细胞外囊泡分析

An Hendrix^1,2, Lien Lippens^1,2,3, Cláudio Pinheiro^1,2,3

¹Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.

Nature reviews. Methods primers

|March 11, 2026

概括

此摘要是机器生成的。

细胞外囊泡 (EVs) 是细胞通信的关键,并具有诊断/治疗潜力. 本书指导了EV分析,方法选择和对各种生物源的解释.

更多相关视频

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Characterizing Extracellular Vesicles from Biological Fluids

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

相关实验视频

Last Updated: May 7, 2026

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Characterizing Extracellular Vesicles from Biological Fluids

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

科学领域:

细胞生物学细胞生物学
生物化学生物化学
生物技术是生物技术.

背景情况:

细胞通过细胞外囊泡 (EVs) 进行通信,这些与脂结合的粒子在健康和疾病中起作用.
电动汽车在诊断和治疗方面表现有前途,在研究中推动了技术进步.
不同的EV隔离和表征方法给研究人员带来了挑战.

研究的目的:

为各种生物来源的细胞外囊泡 (EV) 分析提供指导.
协助研究人员选择适合EV准备和表征的方法.
突出解释EV实验数据的考虑因素,并解决现场限制.

主要方法:

总结了多步骤的EV准备流程.
涵盖了执行和解释EV实验的原则.
讨论各种生态系统的分析,包括身体和环境来源.

主要成果:

提供了对EV分析技术的全面概述.
提供了基于实验需求的方法选择的见解.
解决电动汽车研究中的挑战,包括异质性和环境因素.

结论:

细胞外囊泡 (EV) 研究正在迅速推进,需要仔细的方法选择和解释.
标准化方法和明确的指导方针对于在诊断和治疗中推进EV应用至关重要.
这本入门书是导航电动汽车分析和研究复杂性的资源.