对实验室方法进行废水和环境监测Salmonella的系统审查
Lucky Sangal1, Vishesh Sood2, Karin Haar2
1Immunization & Vaccine Development Unit, World Health Organization South-East Asia (WHO SEARO), New Delhi, India.
Frontiers in public health
|March 11, 2026
概括
对沙门氏菌的废水监测至关重要,但各种实验室方法阻碍了准确的检测. 为了可靠的环境监测和公共卫生洞察,需要标准化的协议.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生监督是对公共卫生的监督.
- 传染病流行病学传染病流行病学
背景情况:
- 废水和环境监测 (WES) 补充了传染病的临床数据,特别是在资源有限的环境中.
- 检测沙门氏菌 spp. 的检测 在废水和地表水中,对于公共卫生监测至关重要.
- 当前WES实践中的方法多样性对标准化提出了挑战.
研究的目的:
- 系统地审查在废水和受污染的表面水中检测沙门氏菌的实验室方法.
- 评估这些实验室方法的多样性,可行性和标准化需求.
- 为了确定当前沙门氏菌 WES 协议中的缺口.
主要方法:
- 在PROSPERO (CRD42024573052) 注册的系统审查,遵循PRISMA 2020指南.
- 在PubMed,EMBASE和Web of Science进行的搜索截至2025年5月31日.
- 包括关于在水表中检测沙门氏菌的研究,不包括非同行评审或非英语出版物;由于异质性而导致的叙事综合.
主要成果:
- 包括来自36个国家的94项研究,采集样本是最常见的方法.
- 培养,PCR和测序是常见的检测技术,但在协议中存在显著的异质性.
- 不到14%的研究报告了全面的质量控制,影响了可复制性和可比性.
结论:
- 对质量控制和验证标准的不一致报告限制了证据的产生.
- 缺乏详细的方法信息阻碍了沙门氏菌WES的复制性和可扩展性.
- 协调,适应环境的协议和最低报告标准对于有效的WES实施至关重要.
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