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核桃谷蛋白抗菌可以通过代谢途径和新型抗菌识别向金黄色葡萄球菌

Hui Ma^1,2, Zhiqiang Lu¹, Han Yang¹

¹College of Food Science and Pharmaceutics, Xinjiang Agricultural University, Urumqi 830002, PR China.

Food chemistry: X

|March 11, 2026

概括

核桃谷蛋白抗菌 (WGAP) 通过破坏细菌的新陈代谢和粘附,有效地对抗抗生素耐药的金黄色葡萄球菌. 这些植物性可以作为传统抗生素的可持续替代品.

科学领域:

食品科学食品科学食品科学
微生物学微生物学
生物化学生物化学

背景情况:

形成生物膜,抗生素耐药的病原体,如金黄色葡萄球菌构成重大挑战.
迫切需要可持续和有效的替代传统抗生素.

研究的目的:

探索核桃谷蛋白抗菌 (WGAP) 作为潜在的抗菌剂.
用多组学和计算方法阐明WGAP对黄金葡萄球菌的作用机制.
从WGAP中识别新的抗菌.

主要方法:

在WGAP中对黄金葡萄球菌进行了有效性和血液溶解的测试.
代谢学和化学被用来了解WGAP对细菌通路的影响.
计算生物学,包括分子对接,用于识别标.
的分离和净化涉及抑制区分析,超过和染色学.

主要成果:

在WGAP的研究中,WGAP表现出对黄金葡萄球菌 (MIC = 53.5μg/mL) 具有显著的抗菌活性,血液溶解率低.
通过WGAP处理,可以降低细菌表面的疏水性,抑制粘附和生物膜的形成.
代谢学表明WGAP会破坏尼古丁胺胺辅因子合成,纯素代谢和TCA循环.
确定了五种新型抗菌,其中DVLINAYR和APQLLYIVK显示强烈的结合于金黄色葡萄球菌内核酶-IV.

结论:

WGAP对黄金葡萄球菌有效,并减少生物膜的形成.
通过损害重要的代谢途径和能量平衡,WGAP具有抗菌作用.
新型WGAP显示出作为向抗菌剂的潜力,为抗生素耐药性提供可持续的植物性解决方案.

关键词:

抗菌是一种抗菌.代谢学代谢学代谢学分子对接是分子对接.黄金葡萄球菌黄金葡萄球菌核桃糖质蛋白质蛋白质蛋白质蛋白质蛋白质蛋白质是核桃糖质蛋白质的组成部分.

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