适应临床化学等离子体作为液体活检的来源
medRxiv : the preprint server for health sciences
|March 11, 2026
概括
常规临床化学测试中的残留血是无细胞DNA (cfDNA) 生物储存和测试的可行来源. 这种可访问的cfDNA资源为分子分析提供了高一致性,支持其在诊断中的使用.
科学领域:
- 分子诊断学 分子诊断
- 基因组学就是基因组学.
- 这是生物银行.
背景情况:
- 循环无细胞DNA (cfDNA) 对于分子测试至关重要,但通常需要专门收集或立即处理.
- 在临床化学中常见的氨酸分离器,是cfDNA分析中未充分利用的来源.
研究的目的:
- 评估使用氨酸分离器的残留血用于cfDNA生物库和测试的可行性.
- 评估从肝素分离器血中获得的cfDNA分析与标准方法 (EDTA,Streck管) 的一致性.
主要方法:
- 分析了来自健康志愿者的匹配血样本和病毒PCR阳性患者队列.
- 实验包括立即处理的比较和模拟的临床处理与延迟处理.
- 分析涉及全基因组和甲基化测序,以评估cfDNA完整性,甲基化模式,碎片组学和病毒检测.
主要成果:
- 在立即处理下,肝素分离器血对甲基化模式和碎片化特征与EDTA和Streck血具有很高的一致性.
- 肝素分离器中的cfDNA完整性在模拟临床处理期间冷藏时与EDTA管相比.
- 在临床队列中观察到病毒检测,复制数改变概况和甲基化模式的强烈一致性.
结论:
- 常规临床化学测试中的残留血,当及时处理和冷藏时,是cfDNA生物库的宝贵和尚未开发的资源.
- 这种方法提高了cfDNA测试和分子诊断的可访问性.
- 这些发现支持将肝素分离器血集成到cfDNA生物银行策略中.
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