在人类CRC队列中检测Clostridioides difficile
medRxiv : the preprint server for health sciences
|March 11, 2026
概括
困难菌常常在结直肠癌 (CRC) 瘤中被检测出来,尽管其数量很少. 它的存在与生物膜阳性组织相关,这表明它在CRC病变发生过程中发挥了作用.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 胃肠病学 胃肠病学
背景情况:
- 肠道微生物组在结直肠癌 (CRC) 病原发生中的作用正在调查中.
- 人类CRC衍生的Clostridioides difficile菌株在小鼠中诱导了结肠瘤发生.
- 这项研究调查了C. difficile对人类CRC发展的贡献.
研究的目的:
- 为了确定C. difficile在人类CRC瘤中的流行率和丰度,与配对的正常组织相比.
- 探索C. difficile检测和瘤生物膜状态之间的关联.
- 分析C. difficile阳性CRC病例中的微生物群体和功能差异.
主要方法:
- 分析了来自108名CRC患者的瘤/正常组织样本.
- 使用16S rRNA测序,培养和PCR检测C. difficile的发现.
- 瘤生物膜存在的评估和与C. difficile检测的相关性.
主要成果:
- 在38%的个体中检测到C. difficile,在瘤 (0.01%) 和正常组织 (0.006%) 中的相对丰度较低.
- 在生物膜阳性瘤中,C. difficile的检测明显更为普遍 (p=0.04).
- 探索性分析揭示了C. difficile阳性和阴性组之间的分类学和功能途径差异.
结论:
- 困难菌常常在CRC瘤微生物群中少量存在.
- 这种细菌可能会显著影响瘤微生物组的组成和功能.
- 研究结果表明,C. difficile在CRC病变发生过程中可能发挥作用,特别是在生物膜相关的瘤中.
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