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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

10.9K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.3K
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
RNA Structure01:19

RNA Structure

8.0K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.0K
RNA Structure01:23

RNA Structure

79.9K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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相关实验视频

Updated: Mar 12, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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一个空间编码策略,用于在现场可视化RNA特异性糖形状.

Ben Niu1, Shuang Xie1, Ruijia Deng1

  • 1Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University, 30 Gaotanyan, Shapingba, Chongqing 400038, China.

Analytical chemistry
|March 11, 2026
PubMed
概括

一种名为SpaceFRET (空间编码的Förster共振能量转移) 的新方法可视化活细胞上特定的RNA糖形. 这项技术识别了甘氨酸-RNA结合,并绘制了甘氨酸RNA分布图,进步了我们对它们功能的理解.

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Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
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Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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相关实验视频

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Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 由于异质性和复杂结构,在活细胞表面上可视化特定位点的RNA糖形具有挑战性.
  • 葡萄糖RNA异质性和共价链接使直接观察变得复杂.

研究的目的:

  • 开发一种用于在现场可视化RNA特异性糖形的新方法.
  • 为了能够识别共价甘氨酸-RNA结合和空间分布分析.

主要方法:

  • 开发了SpaceFRET (空间编码的Förster共振能量传输),集成化学酶性甘氨酸标记 (CeGL) 和近距离诱导的双重编码系统.
  • 使用生物对等化学组,用于FRET供体探针的点击化学,以及用于FRET受体的序列特定的发针探针.
  • 使用链位移反应来解码糖基RNA信息.

主要成果:

  • 成功地可视化了 sia-glycoRNA,Gal/GalNAc-glycoRNA 和 LacNAc-glycoRNA.
  • 在小的细胞外囊泡上阐明了糖核酸RNA的存在.
  • 细胞表面甘氨酸RNAs的划定空间分布及其与脂质的同位化.

结论:

  • 太空FRET提供了一个多功能平台,用于分析RNA特异性糖形.
  • 这种方法对验证甘氨基RNAs的功能机制具有重大前景.
  • 能够在细胞表面和细胞外囊泡上详细地绘制细胞表面和细胞外囊泡上的葡萄糖RNA的空间映射.