分离O-desmethylvenlafaxine和tramadol反体,使用二维合LC×DMS质谱法进行分离
A Dawson McLachlan1, Rashne Vakharia1,2, Emir Nazdrajić1
1Department of Chemistry, Faculty of Science, University of Waterloo, Ontario, Canada. shopkins@uwaterloo.ca.
The Analyst
|March 11, 2026
概括
分离离子移动性谱学与合性LC-MS2相结合,可以有效地分离和量化废水中的O-desmethylvenlafaxine (ODV) 和tramadol (TRA) 的反体. 这种先进的分析方法克服了环境样本中协同排放干扰所带来的挑战.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 法医毒理学 法医毒理学
背景情况:
- 双重质谱 (MS2) 对于选择性分析至关重要,但由于特征卷积而难以量化异构体/异构体.
- 与O-desmethylvenlafaxine (ODV) 抗体一起产生干扰物,如特拉马多尔 (TRA),使环境分析复杂化.
- 状分离对于区分药品和滥用药物的反体是必不可少的.
研究的目的:
- 开发和验证一种方法来分离和量化废水中ODV和TRA的反体.
- 为了应对复杂环境矩阵中协同排放干扰的挑战.
- 评估Chiral LC-DMS-MS2用于药品环境监测的性能.
主要方法:
- 分离离子移动性谱学 (DMS) 与合液体色谱-并联质谱学 (LC-MS2) 的结合.
- 应用开发的方法来分析六个废水流入样本.
- 使用逆相LC-MS2.2.2进行反向相LC-MS2.2.3使用反向相LC-MS2.2.3进行反向相LC-MS2.2.4使用反向相LC-MS2.2.4进行反向相LC-MS2.4.3使用反向相LC-MS2.4.4进行反向相LC-MS2.5.
主要成果:
- 通过合 (LC × DMS) -MS2方法,成功分离和定量了ODV和TRA反体.
- 废水样本中的异构体度与通过逆相LC-MS2获得的异构体度在统计上是相同的.
- 确定了ODV的特定低强度产物离子,使得单独通过奇拉LC-MS2进行量化,尽管量化限制更高.
- 仪器量化极限 (LOQ) 为TRA反体的0.67 ng mL-1和ODV反体的5.0 ng mL-1.
结论:
- 石化LC-DMS-MS2是一种强大的工具,用于解决和量化废水等复杂样品中的反体.
- 这种方法提供了药品及其干扰的准确量化,克服了传统LC-MS2.2的局限性.
- 开发的技术增强了奇拉化合物的环境监测能力.
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