全血储存时间会降低凝块强度,功能性纤维素度的变化最小
Elizabeth R Maginot1, Nicolle K Barmettler1, Flobater I Gawargi1
1Division of Acute Care Surgery, Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA.
全血 (WB) 储存不会降低纤维素原功能,即使在35天后. 白血病减少状态可能会影响结果,但纤维素原仍然具有功能,存储效应可能是由于剩余的血小板造成的.
科学领域:
- 输血医学 输血医学
- 血液静止和血栓形成
- 储存血液 储存血液 储存血液
背景情况:
- 全血 (WB) 是一种关键的复苏液,但其在储存期间对血液静止的影响尚未完全理解.
- 以前的研究表明纤维素原功能下降,但被血小板干扰所混.
- 白血病减少 (LR) 与非白血病减少 (NLR) 对纤维素素保存的影响尚不清楚.
研究的目的:
- 为了研究储存时间对全血纤维素素功能的影响.
- 为了比较白血病减少 (LR) 与非白血病减少 (NLR) 全血中的纤维素素素保存.
- 为了区分储存引起的血液静止变化和与血小板相关的文物.
主要方法:
- 全血单位 (n=15,7 NLR,8 LR) 存储时间长达35天.
- 旋转性血栓形成显微镜 (ROTEM EXTEM) 评估了凝块的动态.
- 纤维素水平是通过西部斑点测量和功能活动通过克劳斯测定在血小板贫血 (PPP) 在结解周期后测量.
主要成果:
- 随着时间的推移,WB储存显示了α角的降低和最大的凝块坚度.
- 血小板数量在第7天显著下降.
- 西方斑点在LR或NLRWB中都没有显示出纤维素降解.
- 克劳斯试验显示,纤维素素功能在第35天得到保存,NLR单位的水平下降趋势不显著.
结论:
- 纤维素素在WB储存期间不会降解,并且在35天内保持功能.
- 白血病减少状态可能会影响纤维素原测定结果,但功能被保留.
- 观察到的静血变化很可能归因于剩余的血小板,而不是纤维素原损失.
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