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低温保护生物墨水使附着细胞的直接生物打印成为可能

Xiyuan Zhao^1,2,3,4, Shenglong Ding⁵, Dadi Sun⁵

¹Human Organ Physiopathology Emulation System, Institute of Zoology, Chinese Academy of Sciences, Beijing, P. R. China.

Advanced materials (Deerfield Beach, Fla.)

|March 11, 2026

概括

结冷保存集成生物打印采用一种新型生物墨水 (CAMP) 来实现冷后细胞的直接3D打印. 这种方法克服了冰损伤,保持了细胞活力,并在体内促进了骨的完整再生.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
再生医学是一种再生医学.

背景情况:

结冷保存集成生物打印为组织再生提供了一个有前途的途径,因为它可以在解后直接打印带有细胞的生物墨水.
关键的挑战包括冰结晶损害细胞活力和水凝的结构完整性,阻碍可打印.

研究的目的:

引入一个双相生物墨水平台,CAMP (Cryopreservation for Adhesion and Maintenance Printing),用于启用冷保存的3D生物打印.
为了克服冰结晶的局限性,并保持细胞和材料的完整性,以便在融化后直接打印.

主要方法:

开发了CAMP,一个生物墨平台,可以在196°C储存后在4-8°C直接进行3D打印.
CAMP利用键介导的水固定来抑制冰的再结晶,通过细胞活力和冰的再结晶抑制试验进行评估.
研究了细胞机制,包括焦点粘附和-FAK信号传递.
使用大鼠大腿缺陷模型在体内评估治疗疗效.

主要成果:

在没有有毒冷保护剂的情况下,CAMP在冷保存后实现了大约80%的细胞活力.
与酸盐缓冲盐水相比,CAMP表现出明显更高的冰再结晶抑制.
在CAMP中冷保存的细胞保留了焦点粘附,并显示酸化FAK的表达增加,抑制细胞死亡.
在体内研究显示,使用CAMP结构,在三个月内,大鼠大腿缺陷的骨完全再生.

结论:

通过整合细胞冷保存,3D生物打印和功能组织形成,CAMP克服了传统生物制造的关键局限性.
该平台为创建即可植入的工程组织提供了一个单一的工作流.
在临床上可行的组织再生解决方案方面,CAMP代表了重大进展.

关键词:

在3D生物打印中使用3D生物打印生物墨水冷保存生物墨水冷保存微载体的微载体是什么组织再生组织再生

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