维护使用四素CD9作为一个入口受体由rhabdoviruses跨越多个属
Wanwan Zhang1,2, Bingbing Sun1, Hao Huang1
1School of Marine Sciences, Sun Yat-sen University, Zhuhai 519082, China.
概括
松素CD9作为一种关键的入口受体,用于各种rhabdoviruses,包括病毒性出血性败血病病毒 (VHSV) 和膀性口腔炎病毒 (VSV). 尼塔佐化物 (NTZ) 通过阻断这种相互作用,显示出作为广泛的抗病毒药物的前景.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 拉布多病毒具有广泛的宿主范围,但使跨物种传播成为可能的细胞受体尚未得到充分理解.
- 识别这些受体对于理解病毒热带性和开发广泛的抗病毒药物至关重要.
研究的目的:
- 为了识别各种rhabdoviruses的保存细胞进入受体.
- 调查四素CD9在拉布多病毒感染和宿主热带性中的作用.
- 探索针对已识别的受体-病毒相互作用的潜在治疗干预措施.
主要方法:
- 利用细胞系和斑马鱼中的CD9淘汰模型来评估病毒感染.
- 进行了病毒葡萄糖蛋白G和CD9细胞外域之间的蛋白质相互作用研究.
- 研究了病毒进入的途径,包括克拉斯林和洞穴介导的内细胞分裂.
- 针对多种rhabdoviruses的广泛抗病毒活性选的化合物.
主要成果:
- 鉴定了四素CD9作为一种维护的功能性入口受体,用于VHSV,SCRV和VSV的不同基因.
- 在VHSV糖蛋白G和Lateolabrax japonicus CD9 (LjCD9) 的细胞外循环之间展示了直接相互作用.
- 证实LjCD9调解VHSV和SCRV的入口,人类CD9作为VSV受体.
- 发现尼塔佐化物 (NTZ) 是一种广谱抑制剂,可能通过破坏G蛋白-CD9相互作用.
结论:
- 拉斯巴宁CD9是各种rhabdoviruses的保存,跨物种的入口受体.
- LjCD9促进了VHSV通过克拉特林和洞穴介导的内细胞分裂进入.
- 尼塔佐化物 (NTZ) 是一个有前途的宽谱抗病毒药物,向CD9介导的进入途径.
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