一个动态的Dif/lncRNA-CR42715/miR-965-3p反循环在Drosophila中编排了收费通路免疫反应
Xiaolong Yao1,2, Lin Zhou1, Rui Wu1
1Laboratory of Molecular Immunology and Bioinformatics & Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China.
PLoS pathogens
|March 11, 2026
概括
一个新的反循环,涉及长非编码RNA (lncRNA) 和微RNA (miRNA),对果进行微调.
科学领域:
- * 免疫学和分子生物学研究重点是Drosophila的先天免疫调节.
- *研究涉及非编码RNA (ncRNA) 的转录后调节网络.
背景情况:
- * Drosophila Toll 途径对先天免疫至关重要,但其转录后调节尚未完全理解.
- *非编码RNAs (ncRNAs) 在生物过程中发挥重要作用,包括免疫反应.
- *了解调节机制是防止免疫过度激活和确保有效清除病原体的关键.
研究的目的:
- *阐明了一种新型的调节反循环,涉及Drosophila Toll信号通路中的ncRNA.
- * 描述lncRNA CR42715,miRNA miR-965-3p和转录因子Dif在免疫反应调节中的作用.
- * 了解这种循环如何动态调节免疫信号,以有效地防御病原体.
主要方法:
- *利用基因操纵来破坏反循环组件 (CR42715和miR-965-3p).
- * 在感染期间对关键调节分子进行了时间表达分析.
- *研究了竞争性内源RNA (ceRNA) 机制,涉及lncRNA,miRNA和转录因子.
主要成果:
- * 确定了三方反循环:Dif激活了CR42715,这使miR-965-3p成为海绵,从而增强了Dif蛋白质的合成.
- *循环中断导致抗微生物 (AMP) 表达失调,宿主存活率降低.
- *CR42715在感染早期迅速诱导,而miR-965-3p在晚些时候增加以减弱信号.
结论:
- * 一个新型的转录因子-lncRNA-miRNA反循环作为一个静电器来调节Drosophila Toll信号传输.
- *这种调节机制确保了强大的早期免疫反应,同时防止过度晚期免疫.
- *这些发现为通过ncRNA介导的反循环对先天免疫的动态控制提供了洞察力.
相关概念视频
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Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
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MHC Class I: Presenting Endogenous...
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