在Bacillus subtilis中Tfcut-B3的直接演变和表达,以有效地去聚合商业PBAT基塑料薄膜
Mingliang Zhang1, Fanghui Ding1, Xi Xiao1
1Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
Journal of hazardous materials
|March 11, 2026
概括
一种新型突变酶,Tfcut-B3,有效地去聚合聚乙烯酸盐-共甲 (PBAT) 薄膜,产生比其前身多得多的甲酸 (TPA). 这一突破推动了PBAT废物的酶回收.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 环境科学 环境科学
背景情况:
- 酶去聚合提供了一种可持续的回收方法,用于回收聚乙烯基酸盐-联合甲酸盐 (PBAT) 废物.
- 已知酶对各种商业PBAT薄膜的脱聚合效率尚未确立,这限制了实际应用.
研究的目的:
- 开发一种改进的聚乙烯酸盐-共甲酸盐 (PBAT) 脱聚合酶,具有增强的热稳定性和活性.
- 为了评估工程酶的脱聚合能力与各种商业PBAT膜相比.
主要方法:
- 使用定向进化来从Tfcut-DM中创建一个突变的PBAT脱聚合酶,Tfcut-B3.
- Tfcut-B3的脱聚合活性在PBAT白膜和十个商业PBAT基膜上进行了测试.
- 在Bacillus subtilis中实现了Tfcut-B3的过度表达和细胞外分泌,随后进行了小瓶发酵.
主要成果:
- 在48小时内,TFCUT-B3将PBAT白薄膜完全脱聚合成铁酸 (TPA),分别产生的TPA是TFCUT-DM和TFCUT-DM的2.0倍和1.3倍.
- 在48小时内,Tfcut-B3成功将10个商业PBAT片中的8个降解为TPA.
- 在Bacillus subtilis中通过促进体和宿主优化实现了274 mg/L Tfcut-B3的高表达水平.
结论:
- 与以前的酶相比,工程Tfcut-B3酶显示了PBAT膜的优越脱聚合能力.
- 在Bacillus subtilis中成功过度表达和分泌功能Tfcut-B3,为大规模生产铺平了道路.
- 这些发现支持Tfcut-B3在有效和环保的PBAT废物的酶循环利用方面的潜力.
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