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该MaEIL9-MaZIP5-MaSCL8模块集成MaBEL1并协同调节香果的成熟过程

Fan Liu¹, Xueli Sun², Ou Sheng²

¹Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Science and Technology Research on Fruit Tree, Guangzhou 510510, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.

Journal of advanced research

|March 11, 2026

概括

一项研究显示,GRAS基因MaSCL8通过激活参与乙烯生产和水果软化关键基因,积极调节香成熟. 这项研究增强了对水果发育中的植物转录因子的理解.

科学领域:

植物分子生物学植物分子生物学
开发水果的水果开发
转录因子法规转录因子法规

背景情况:

果实成熟是一个复杂的,基因控制的过程,涉及复杂的基因调节网络.
草 (GAI-RGA-和-SCRs) 转录因子对植物生长和发育至关重要,但它们在水果成熟中的作用尚不清楚.

研究的目的:

研究 GRAS 基因 MaSCL8 在控制香果成熟过程中的调控机制.
阐明涉及MASCL8.8的上游和下游监管途径.

主要方法:

酵母单混合体,双露西法酶记者和电泳性移动性转移试验被用于识别MASCL8的标和调节者.
路西法酶补充成像和酵母双杂交试验确定了MASCL8-相互作用蛋白.
通过稳定的番茄过度表达和香过度表达进行了功能分析.

主要成果:

马斯克L8作为核转录激活剂,积极调节香的成熟.
MaSCL8直接增强了参与乙烯生物合成,粉降解和细胞壁修饰 (例如,MaAMY3,MaACO1) 的基因的转录.
MaSCL8与MaBEL1相互作用,促进目标基因转录,MaEIL9-MaZIP5级联激活了MaSCL8的表达.

结论:

该MaEIL9-MaZIP5-MaSCL8模块,与MaBEL1一起,推动香的成熟.
这种调节模块增强了乙烯生产,粉降解和细胞壁松动.
这些发现大大提高了对GRAS介导的水果成熟机制的理解.

关键词:

香香香是一种果实成熟的时间互动互动互动互动马斯克L8 公司转录法规转录法规

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