一个主要的因素是中国树丝虫Antheraea pernyi中白色素介导的蓝色色素的基础
Xiaojing Liu1, Zhongjie Zhang1, Han Gao1
1Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China; Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China.
Insect biochemistry and molecular biology
|March 11, 2026
概括
研究人员在中国木丝虫Antheraea pernyi.中发现了六个胆素结合蛋白 (BBP) 基因. 一种特定的BBP基因删除解释了一个菌株中没有蓝绿色的幼虫皮肤色素.
科学领域:
- 昆虫色素遗传学 昆虫色素遗传学
- 昆虫颜色的生物化学 昆虫颜色的生物化学
- 发育生物学是发展生物学.
背景情况:
- 昆虫幼虫的颜色对生态和发育至关重要,涉及复杂的途径.
- 胡卜素和黄素是已知的颜料,但蓝绿色白素颜色的分子基础尚不清楚.
- 中国木丝虫 (Antheraea pernyi) 显示出自然的颜色变化,作为颜色研究的模型.
研究的目的:
- 为了确定负责Antheraea pernyi.在 biliverdin 相关色素的基因.
- 阐明蓝皮 (Lan) 和白皮 (Bai) 菌株之间的颜色差异背后的遗传机制.
- 建立Antheraea pernyi作为研究色素进化和调节的模型.
主要方法:
- 发现和描述六个胆白素结合蛋白 (BBP) 基因 (ApBBP1-6).
- 综合转录和qRT-PCR分析以评估基因表达模式.
- 基因组比较以确定菌株之间的遗传变异.
- 在Lan菌株中进行了Cas9/sgRNA介导的基因切除实验.
主要成果:
- 鉴定了6个ApBBP基因;在蓝色Lan菌株的表皮,中肠和体脂肪中,ApBBP1的表达高,但在白色Bai菌株中没有.
- 在Bai菌株的ApBBP1位点中~3.9kb的删除导致了截断的编码序列.
- 在兰氏菌株中,对ApBBP1的基因切除显著降低了mRNA水平和比利弗丁含量,使皮肤变白.
- 代谢物分析证实,在去除后,比利韦丁水平降低了.
结论:
- 这项研究确定ApBBP1是Antheraea pernyi幼虫中由白色素衍生的蓝绿色染色体的关键基因.
- 在ApBBP1中有一种特定的缺失解释了白色皮肤白色菌株缺乏色素的原因.
- 这一发现为昆虫色素的演变提供了洞察力,并为工程昆虫颜色提供了潜力.
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