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miR-146a/b-5p-IRAK1-NF-κB轴调节无法解释的反复性自发流产

Fengping He¹, Shushu Fan², Lujing Chen¹

¹Institute of Reproduction and Genetics, Dongguan Maternal and Child Health Care Hospital, Dongguan, Guangdong, China.

Journal of molecular recognition : JMR

|March 11, 2026

概括

微RNAs miR-146a/b-5p通过向IRAK1并调节NF-κB通路来调节无法解释的复发性自发性流产 (URSA). 这一发现表明miR-146a/b-5p是URSA的潜在治疗标.

科学领域:

产科和妇科产科和妇科
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

无法解释的复发性自发性流产 (URSA) 是一种常见的产科并发症,原因不明.
微RNAs (miRNAs) 参与调节基因表达,并且可能在URSA病变发生过程中发挥作用.

研究的目的:

为了确定参与URSA的关键miRNAs.
阐明URSA中这些miRNAs的调节机制.
探索URSA的潜在治疗点.

主要方法:

对GEO数据集 (GSE178619,GSE113790) 的生物信息分析,以识别差异表达的miRNA和mRNA.
权重基因共同表达网络分析 (WGCNA) 以确定枢纽基因和miRNAs.
在体外研究中使用氧化低密度脂蛋白 (OX-LDL) 诱导的热囊细胞模型.
使用URSA小鼠模型进行体内研究.
对miR-146a/b-5p和IRAK1.1的功能增益和丧失实验
双露西法酶记者测定.
对NF-κB信号通路激活的评估.

主要成果:

在URSA中确定了差异表达的miRNA和mRNA.
确定了miR-146a/b-5p和IRAK1作为关键分子,IRAK1在URSA中被上调.
OX-LDL 损害了 trofhoblast 功能,减少了 miR-146a/b-5p,并通过 IRAK1.1 激活了 NF-κB.
在小鼠中,miR-146a/b-5p过度表达改善了OX-LDL诱导的损伤,改善了怀孕结果.
miR-146a/b-5p直接准IRAK1,调节NF-κB通路. 这样一来,我们可以直接控制IRAK1.

结论:

miR-146a/b-5p通过准IRAK1和调节NF-κB信号通路,在URSA中发挥着至关重要的作用.
miR-146a/b-5p代表了管理URSA的潜在治疗标.

关键词:

伊拉克1伊拉克1伊拉克通过NF-κB路径.在 miR-146a-5p 中,在 miR-146b-5p 中,无法解释的复发性自发性流产

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...