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用"IEDDA"激活的光传感器检测蛋白质氨酸氧化还原变化,并对蛋白质氨酸氧化还原变化进行分析

Linfeng Chen¹, Yifu Zheng¹, Ziyi Wang¹

¹National Key Laboratory of Green Pesticide, College of Chemistry, International Joint Research Center for Intelligent Biosensing Technology and Health, Central China Normal University, Wuhan, 430079, China.

Analytica chimica acta

|March 11, 2026

概括

研究人员开发了一种新的"启动"光探针策略,用于跟踪蛋白质氨酸氧化还原变化. 这种方法在细胞和生物体中提供了高对比度成像,推进了氧化还原生物学研究.

科学领域:

降解毒素生物学和化学生物学
生物对等化学和成像技术

背景情况:

蛋白质氨酸硫化是氧化还原信号和疾病中的关键可逆变化.
现有的硫化光探针具有局限性,包括高背景信号和无法实时跟踪.

研究的目的:

开发一种无背景的"开启"光传感策略,用于监测蛋白质氨酸的氧化还原变化.
为了实现活跃的,实时可视化活体系统中囊氧化还原事件.

主要方法:

设计的选择性探头 (BCN-SH和 BCN-SOH) 使用bicyclo[6.1.0]non-4-yne (BCN) 柄用于标记蛋白质硫醇和硫酸.
使用反向电子需求迪尔斯-阿尔德 (IEDDA) 反应,用基于四素的光体 (BODIPY-tetrazine, BTZ) 进行生物对等激活.
在活的HeLa细胞和Caenorhabditis elegans中验证了探针的选择性,稳定性和性能.

主要成果:

在IEDDA反应时实现了248倍的光增强,从而实现了高对比度检测.
在氧化应激下,成功地在活的HeLa细胞中可视化了内源性氨酸氧化还原动力学.
证明了该策略在Caenorhabditis elegans的光成像中的适用性.

结论:

开发的平台集成了选择性氨酸化学与原生物对等激活,以免背景可视化动态氧化还原事件.
这一策略扩大了氧化还原生物学的化学工具箱,促进了氧化信号通路的机制研究.

关键词:

生物对等化学的化学.囊氨酸的氧化方式这就是IEDDA的反应.活细胞成像成像技术重氧化成像成像复氧化成像

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