自动交叉连接的子丝纤维促进了内和Cry毒素的聚合
Mike Sleutel1,2, Adrià Sogues3,4, Han Remaut5,6
1Structural and Molecular Microbiology, VIB-VUB Center for Structural Biology, VIB, Brussels, Belgium. mike.sleutel@vub.be.
Nature communications
|March 12, 2026
概括
修灵菌 (Bacillus thuringiensis) 使用一种由α-endospore附属物 (A-ENA) 组成的新型"体丝"矩阵,以增强其杀虫活性. 这种蛋白质涂层聚合了子和毒素,增加了毒性,并为非转基因生物害虫控制应用提供了潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 细菌 thuringiensis (Bt) 是一种用于生物害虫控制的细菌,产生杀虫毒素.
- 它的有效性取决于感染昆虫幼虫的子和体 (PSBs).
- 不过,Bt子和PSB的表面结构及其对毒性的贡献尚未完全理解.
研究的目的:
- 为了描述覆盖Bacillus thuringiensis Sv. 的蛋白质质矩阵. 以色列的子和PSBs.
- 调查这个矩阵作为毒性因子的结构,组合和功能.
- 探索这个矩阵在增强生物害虫防治剂方面的潜力.
主要方法:
- 电子显微镜可视化"体丝"矩阵及其纤维结构.
- 生物化学分析以确定α-内胞附属体 (A-ENA) 的组成和稳定性.
- 重组蛋白质生产和A-ENA的自我组装研究.
- 功能性试验评估A-ENA对Bt毒性和PSB子聚类的影响.
主要成果:
- Bt子和PSB被覆盖在由A-ENA组成的8纳米宽的双螺旋"子丝"矩阵中.
- A-ENA纳米纤维由多个分子间异酸键稳定,形成一个强大的聚合物.
- A-ENA通过聚合子和PSB作为毒性因子,增强杀虫活性.
- 再组合的A-ENA可以自组装成纤维,并且在外源添加时,可以诱导Bt菌株的聚类并增强缺乏本地菌丝菌株的毒性.
结论:
- 由A-ENA形成的"子丝"矩阵是Bacillus thuringiensis毒性的关键结构组成部分.
- 由于A-ENA能够将传染性颗粒聚集在一起,这显著提高了杀虫剂的有效性.
- 这些发现使得Bacillus thuringiensis的合理,非转基因功能化能够改善生物害虫控制.
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