集成的小和长RNA测序揭示了在人类 oogenesis 期间piRNA介导的转子子抑制
Fengjuan Zhang1, Hongdao Zhang2, Yali Xiao1
1State Key Laboratory of RNA Innovation, Science and Engineering, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
Nature communications
|March 12, 2026
概括
人类的 oogenesis 依赖于 piwi 相互作用 RNAs (piRNAs) 来控制可转移元素. 短的piRNA在很大程度上抑制了TE表达,而长的piRNA则提供了对内源性逆转录病毒的特定抑制.
科学领域:
- 生殖生物学 生殖生物学
- RNA生物学的RNA生物学
- 基因组学就是基因组学.
背景情况:
- 交互RNAs (piRNAs) 对于生殖细胞的基因组完整性至关重要.
- 它们在人类 oogenesis 中的精确作用和表达模式尚未完全理解.
研究的目的:
- 在人类 oogenesis 期间调查小和长RNA 的动态和功能.
- 阐明不同PIWI/piRNA类在可转移元素 (TE) 抑制中的作用.
主要方法:
- 在四个发育阶段的个人人体卵细胞中,同时对小RNA和长RNA进行分析.
- 对piRNA集群及其插入偏差的基因组分析.
主要成果:
- 短的piRNAs,特别是PIWIL3相关的piRNAs,在人类生殖过程中占主导地位,并且大大降低了TE表达 (LINE-1,ERVs).
- 长的piRNAs (PIWIL1/2-关联) 与特定的内源性逆转录病毒 (ERV) 亚家族下调相关.
- piRNA集群表现出不对称的插入偏差,针对LINE-1和ERV进行家族特定调节.
结论:
- 短的piRNAs在人类的 oogenesis 期间起到主要的作用,作为TEs的宽谱抑制剂.
- 长的piRNAs提供协调的,ERV特定的抑制,补充短的piRNA功能.
- 这项研究提供了单细胞RNA联合表达数据集,详细介绍了PIWI/piRNA在生殖细胞发育中的作用.
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