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CasY7:一个优化的Cas12i系统,用于在单种植物中进行增强的基因组编辑

Dating Zhong¹, Yanxiao Dong¹, Hong Pan²

¹School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Journal of integrative plant biology

|March 12, 2026

概括

新的CasY7基因编辑工具在玉米,大米和小麦中显示出高效率. 这种CRISPR-Cas12变体显著提高了植物的基因组编辑能力.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
基因编辑技术基因编辑技术

背景情况:

CRISPR-Cas12核酶,特别是Cas12i亚型,为植物基因组编辑提供了潜力.
之前开发的Cas12i变种CasY7在临床试验中取得了成功,但需要进行植物性能评估.

研究的目的:

为了研究CasY7基因编辑系统在植物中的性能.
加强CasY7的活性,并评估其在主要作物物种中的效率和适用性.

主要方法:

优化CasY7 (pCasY7e1) 的编码和对转基因玉米和大米进行测试.
融合T5外核酶到CasY7 (pCasY7e2) 和整合MS2胺体 (pCasY7e3) 来增强编辑.
在玉米,大米和六倍体小麦中验证优化的pCasY7e3系统,包括多重编辑.

主要成果:

编码优化的CasY7 (pCasY7e1) 在玉米中实现了58.7%的平均编辑效率,在大米中达到62.3%.
改进的pCasY7e3系统提高了编辑效率,在玉米中达到87.7%,在大米中达到82.9%,超过了pCpf1.1.
在玉米中成功进行多重编辑,在小麦中进行验证,在942种转基因植物中高达58.8%的效率.

结论:

CasY7是一种高效的CRISPR工具,用于在玉米,大米和小麦中进行基因组编辑.
优化的CasY7系统,特别是pCasY7e3,与标准方法相比,显著改善了编辑结果.
CasY7是CRISPR工具包中一个有价值的补充,用于推进植物生物技术.

关键词:

克里斯普尔卡斯12i 这就是T5外核酶.优化crRNA的优化方法基因组编辑基因组编辑单植物作物单植物作物

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