[脱方法对Cinnamomi Cortex-Coptidis Rhizoma supramolecular的相位状态和生物活性的影响]
Ran Xu1, Zhi-Jia Wang1, Xiao-Yu Lin1
1School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
在Cinnamomi Cortex-Coptidis Rhizoma的共同开制中,可以创建稳定的超分子系统,具有增强的柏柏林和丁酸含量. 与机械混合相比,这种方法产生了对黄金葡萄球菌的优越抗菌活性.
科学领域:
- 超分子化学 超分子化学
- 传统中国医学 (TCM) 药理学 药理学
- 草药相互作用 草药相互作用
背景情况:
- 肉皮层 - - 科普蒂迪斯树脂草药对经常用于中国传统医学 (TCM).
- 了解这些草药之间的相互作用对于优化治疗疗效至关重要.
- 研究不同的制备方法可以揭示相位和生物活性的显著差异.
研究的目的:
- 为了研究Cinnamomi Cortex-Coptidis Rhizoma的高分子化学和草药相互作用.
- 为了比较相位和生物活性之间的差异,共和机械混合方法.
- 阐明制方法如何影响超分子系统的形成和生物活性.
主要方法:
- 宏观观测和度测量用于相位分析.
- 异热定位热度计,紫外线和红外线光谱仪用于草药相互作用分析.
- 动态光散射和SEM用于粒子大小的确定.
- 稀释,板涂层,活/死染色,以及用于抗菌活性评估的SEM.
- 用于组件分析的UPLC-MS/MS.
主要成果:
- 配开导致更稳定的宏观相位状态和足够的分子相互作用.
- 观察到明显的超分子结构,共产生更小,更集中的粒子大小.
- 同时煮沸剂显著增加了柏柏林和丁酸的含量.
- 与一起观察到对黄金葡萄球菌 (Staphylococcus aureus) 的增强抑制活性.
结论:
- 在Cinnamomi Cortex-Coptidis Rhizoma的共同中,可以产生更加均的超分子系统.
- 配开方法提高了主要活性成分的含量,如柏柏林和科普蒂辛.
- 与机械混合相比,共导致对黄金葡萄球菌的抗菌有效性更高.
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