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aGPCR-HEK:一种稳定的高表达可诱导的哺乳动物细胞表达系统用于粘附GPCR结构生物学应用

David M Favara1,2,3, Christopher G Tate1

  • 1Division of Structural Studies, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK.

Bio-protocol
|March 12, 2026
PubMed
概括

研究人员开发了aGPCR-HEK,这是一种新的方法,用于在哺乳动物细胞中产生正确折叠的粘附G蛋白结合受体 (aGPCR). 这个系统可以在毫克尺度上净化aGPCRs,用于诸如冷电子显微镜 (cryo-EM) 等结构研究.

关键词:
粘附性GPCR (aGPCR) 的使用在HEK293S GnTI- TetRR中使用.可以诱导的表达式.林氏病毒 (Lentivirus) 是一种隐形病毒.一个稳定的细胞系.催产四环素诱导作用

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