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Updated: Mar 13, 2026

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings GCMR in the Insect Antennal Lobe

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings GCMR in the Insect Antennal Lobe

Published on: February 24, 2013

宿主-挥发性结合的结构和功能基础鱼 (Lepidoptera: Tortricidae) 中的天线气味结合蛋白

Tengda Guo^1,2, Yumeng Huang¹, Xueqing Yang³

¹State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, 100193 Beijing, China.

Journal of agricultural and food chemistry

|March 12, 2026

概括

鱼的嗅觉依赖于臭剂结合蛋白 (OBPs). 研究人员确定了CpomOBP9和CpomOBP18,详细介绍了它们的特定挥发物结合和在宿主检测中对害虫控制策略的作用.

科学领域:

昆虫嗅觉研究昆虫嗅觉研究
分子昆虫学分子昆虫学
病虫害管理科学病虫害管理科学

背景情况:

(Cydia pomonella) 是一个重要的农业害虫.
嗅觉对于 codling moth 寻找宿主和产卵行为至关重要.

研究的目的:

为了研究两种天线臭剂结合蛋白 (OBPs),CpomOBP9和CpomOBP18在鱼宿主-挥发性识别中的功能.
阐明OBPs在化学传感中的作用背后的分子机制.

主要方法:

使用光竞争性结合试验来测试与27种宿主挥发性物质的相互作用.
用定位突变和RNA干扰 (RNAi) 来确定关键的结合残留物并确认体内功能.
进行了天线电天线图 (EAG) 和Y管嗅法测试.

主要成果:

CpomOBP9对乙烯六酸盐具有很高的亲和力,而CpomOBP18则优先结合α-farnesene.
特定的氨基酸残留物 (CpomOBP9中的Lys91;CpomOBP18中的Ile32,Leu35,Phe49,Leu50) 被确定为关键的联结.
通过RNAi介导的OBP的淘汰显著削弱了对各自挥发物的吸引力.

结论:

这项研究提供了一个结构和功能理解的OBP介导的挥发性物质检测在.
对OBP角色的机械洞察力可以为开发基于半化学的新型害虫控制方法提供信息.

关键词:

RNA干扰 (RNAi) 是一种RNA干扰.codling moth 鱼鱼鱼鱼鱼鱼光竞争性结合试验.宿主植物的挥发性物质气味剂结合蛋白质是气味剂结合的蛋白质.在位点导向的突变发生.

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