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Updated: Jul 29, 2026

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
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从人体血中进行大规模,高产量,高纯度的细胞外囊泡净化方案

Saba Zanganeh1, Gabriel F Arias2, Allaura S Cone3

  • 1Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

STAR protocols
|March 12, 2026
PubMed
概括

这项研究引入了一种新的,可扩展的方法,可以在没有超离心的情况下从人体血中净化细胞外囊泡 (EV),从而提高它们作为生物标志物和药物输送载体的潜力.

关键词:
生物技术和生物工程材料科学 材料科学分子生物学分子生物学蛋白质的生物化学 蛋白质的生物化学蛋白质的表达和净化过程

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相关概念视频

Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...