一个新的微生理学平台,研究神经活性剂穿过肠道和大脑屏障的透
Barbara Pavan1, Giada Botti2, Alessandro Dalpiaz3
1Dept. of Neurosciences and Rehabilitation, University of Ferrara, Via L Borsari, 46, Ferrara, Ferrara, 44121, Italy.
Biofabrication
|March 12, 2026
概括
尤根醇在体外有效地穿越血脑屏障 (BBB) 和血脑脊髓液屏障 (BCSFB),证明了其在中枢神经系统药物输送方面的潜力. 这项研究验证了用于临床前药物查的微生理系统.
科学领域:
- 药理学 药理学是指药理学的学科.
- 神经科学是一个神经科学.
- 生物技术是生物技术.
背景情况:
- 在体外复制血脑屏障 (BBB),血脑脊髓液屏障 (BCSFB) 和肠道屏障 (IB) 等生理障碍对于药物开发至关重要.
- 已知尤根醇在体内透到中枢神经系统 (CNS),使其成为研究屏障透性的相关化合物.
研究的目的:
- 通过微生理学系统在模拟的BBB,BCSFB和IB中评估欧原醇和胆醇的体外透性.
- 评估eugenol在模拟中枢神经系统环境中的多巴胺基神经元上的神经活性.
- 验证CELLBLOKS®和NANOSTACKSTM平台用于临床前药物查和中枢神经系统向研究.
主要方法:
- 利用CELLBLOKS®模块化微生理系统培养人类大脑微血管内皮细胞hCMEC/D3,人类视网膜色素上皮细胞 (HRPE) 和老鼠小肠IEC-6细胞.
- 在CELLBLOKS®系统中模拟输入静脉和口服欧原醇和胆醇的使用.
- 使用高性能液体染色学 (HPLC) 进行透度测量.
- 在NANOSTACKSTM插件上嵌入了类似神经元的多巴胺基PC12细胞,以评估下游的神经活性.
主要成果:
- 在模拟的口服和全身管理后,Eugenol在模拟的BBB和BCSFB中表现出显著的透.
- 与eugenol相比,CELIPROL在BBB和BCSFB中显示出有限的透.
- 尤根醇诱导了BBB和BCSFB受界区的PC12细胞显著释放多巴胺,表明神经活性.
结论:
- CELLBLOKS®和NANOSTACKSTM平台被验证为具有成本效益,可复制和易于使用的工具,用于药物脑向体内研究.
- 这些体外模型显示出减少对中枢神经系统作用药物的临床前动物试验的需求的前景.
- 这项研究强调了eugenol在中枢神经系统药物递送应用中的潜力.
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