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Updated: May 13, 2026

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通过葡萄糖-δ-乳介导聚合和酶交叉链接的Deccan大麻蛋白质基水凝形成:特征和体外释放:
Dibya Ranjan Dash1, Sushil Kumar Singh1
1Department of Food Process Engineering, National Institute of Technology, Rourkela, Odisha, 769008, India.
德坎大麻种子蛋白质水凝是使用葡萄糖-δ-乳 (GDL) 和NaCl来提高结构完整性的. 这些植物蛋白质水凝对封装和输送食品和营养保健品中的生物活性化合物充满希望.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物材料工程 生物材料工程
- 营养药物开发 营养药物开发
背景情况:
- 植物蛋白质水凝正在作为生物活性化合物的载体获得吸引力.
- 德坎大麻种子蛋白 (DHSP) 为水凝发育提供了一个新的来源.
- 优化水凝结构对于有效的封装和输送至关重要.
研究的目的:
- 开发和描述来自Deccan大麻种子蛋白 (DHSP) 的新型水凝.
- 研究葡萄糖-δ-乳 (GDL),NaCl和乳糖酶对DHSP水凝的增强作用.
- 评估DHSP水凝在封装和输送生物活性化合物的潜力.
主要方法:
- 通过使用GDL (1-2%),NaCl (0.2-0.5M) 和laccase (0.5-1 U/g) 来形成DHSP水凝.
- 通过光透度,粒子大小分析,醇/氨基群含量,储存模量 (G'),硬度和弹性来评估水凝的结构性质.
- 微观结构使用共聚焦激光扫描显微镜 (CLSM) 和场发射扫描电子显微镜 (FESEM) 进行可视化.
- 在模拟的胃和肠道条件下,评估了鲁丁的封装效率和释放动力学.
主要成果:
- GDL和NaCl通过结,疏水性和静电相互作用促进了DHSP的聚合和凝.
- 乳糖酶辅助交联显著增强了水凝的结构完整性,由减少的醇和自由氨基基团证明.
- 增加的GDL和NaCl度改善了储存模量 (G') 和结构恢复.
- 在模拟的肠液中,DHSP水凝表现出优异的鲁丁封装效率 (94.78%) 和受控释放 (> 86%).
- 水凝在模拟的胃液中表现出强大的保护,防止降解.
结论:
- 基于DHSP的水凝,以GDL,NaCl和laccase进行优化,提供卓越的结构性能.
- 这些水凝为保护敏感生物活性化合物提供了可调节和有效的系统.
- DHSP水凝是食品和营养品行业有望的有针对性的传递载体.
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