相关实验视频

Updated: Mar 14, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

发现了SARS-CoV-2抗体,这些抗体可以有效地中和Omicron变体

Seth J Zost^1,2, Naveenchandra Suryadevara¹, Lauren E Williamson^1,2

¹The Vanderbilt Center for Antibody Therapeutics, Vanderbilt University Medical Center, Nashville, TN, USA.

Nature microbiology

|March 13, 2026

概括

新的糖甘蒙面抗原使得能够发现强大的单克隆抗体 (mAbs),可以中和多种SARS-CoV-2 Omicron变体. 这种方法针对特定的病毒部位,克服抗体逃逸以获得有效的治疗开发.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
药物发现药物发现药物发现

背景情况:

SARS-CoV-2 Omicron 变种迅速演变,导致单克隆抗体 (mAb) 逃逸.
循环变异中的抗原替代复杂化了治疗mAb的发现.

研究的目的:

开发一种用于快速发现和表征针对SARS-CoV-2变种的mAbs的新方法.
为了分离具有强大的中和活性的mAbs对分离的Omicron亚变体进行中和.

主要方法:

使用甘氨酸掩盖抗原进行以突为重点的mAb发现.
303 mAbs. 的隔离和表达.
皮层图谱绘制和交叉反应性分析.

主要成果:

确定了有效中和分歧的Omicron亚型的mAbs.
针对SARS-CoV-2受体结合域 (RBD) 上的3类抗原位点.
证明了3类网站的交叉反应和差异识别的频谱.

结论:

糖甘蒙蔽的抗原对于有针对性的mAb发现是有效的.
这种策略产生具有广泛中和活性的mAbs,对抗SARS-CoV-2变种.
抗原的合理设计有助于隔离强效的治疗抗体.

更多相关视频

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

相关概念视频

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

Cross-reactivity

Cross-reactivity