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表观遗传调节对从Embelia肋骨中分离出的内细胞的代谢物的影响

Ajinkya Terkar¹, Aditya Raut¹, Jayant Kulkarni¹

¹Department of Botany, Savitribai Phule Pune University, Pune, 411007, India.

International microbiology : the official journal of the Spanish Society for Microbiology

|March 13, 2026

概括

表观遗传修饰剂阿扎西提丁 (AZ) 和丁酸盐 (SB) 成功激活了药用植物真菌内细胞的静态代谢物生产. 这种方法增强了用于生物医学应用的新生物活性化合物的发现.

科学领域:

微生物学和生物技术
自然产品化学自然产品化学
药用植物研究药用植物研究

背景情况:

在药用植物中存在的真菌内细胞是生物活性代谢物的丰富来源.
表观遗传修饰剂可以释放内细胞中神秘的生物合成基因集群的潜力.
了解内细胞代谢学对于发现新的治疗剂至关重要.

研究的目的:

调查表观遗传修饰剂阿扎西丁 (AZ) 和乙酸盐 (SB) 对真菌内生菌的代谢物概况的影响.
阐明Phomopsis azadirachtae和Diaporthe phaseolorum中静态代谢物的激活和二次代谢的改变.
评估表观遗传调节的潜力,以从Embelia ribes的内细胞中发现新的代谢物.

主要方法:

从Embelia肋骨中分离和培养的真菌内植物Phomopsis azadirachtae和Diaporthe phaseolorum.
用不同度的阿扎西提丁 (AZ) 和甲酸盐 (SB) 处理内植物培养物.
使用液体染色学-质谱学 (LC-MS) 进行代谢组分析,以识别和量化代谢物.

主要成果:

在P. azadirachtae和D. phaseolorum中,AZ和SB治疗显著改变了代谢物概况.
激活了多个以前沉默的代谢物,包括迪赛兰德罗尔B,福莫辛A和福莫菲林A.
特定代谢物表现出增强的产量,证明了表观遗传调制在推动二次代谢中的有效性.

结论:

表观遗传修饰剂AZ和SB有效地改变了真菌内细胞中的二次代谢物概况.
这些调节器激活无声的生物合成途径,为新型代谢物发现提供了有前途的战略.
建议进行进一步的多主题研究和结构验证,以充分探索这些发现的潜力.

关键词:

它们是内植物 (Endophyte).表观遗传调制是一种表观遗传调制.代谢物的代谢物.

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