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摄入的纤维素纳米材料对肠道细胞中DNA甲基化的影响

Nádia Vital^1,2,3, Célia Ventura^1,3, Camila Fernandes¹

¹Department of Human Genetics, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016, Lisbon, Portugal.

Particle and fibre toxicology

|March 13, 2026

概括

纤维素纳米材料 (CNMs) 可以改变肠道细胞中的DNA甲基化,其影响因消化而有所不同. 表观遗传学分析对于理解CNM毒性机制至关重要.

科学领域:

纳米材料科学科学纳米材料科学
毒理学毒理学毒理学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

纤维素纳米材料 (CNMs) 正在开发中,用于生物医学和食品应用.
通过口服接触CNM需要对潜在的人类健康影响进行评估.
之前的研究表明,对CNF-TEMPO和CMF-ENZ存在遗传毒性问题.

研究的目的:

研究CNM对肠道细胞的表观遗传作用.
为了探索DNA甲基化在体外模拟消化和CNM暴露后的变化.
评估下游生物效应,而不是通过基因毒性测试捕获.

主要方法:

将体外模拟消化与表观遗传学结合起来.
将肠道Caco-2细胞暴露在未消化的和已消化的CNF-TEMPO和CMF-ENZ中.
使用减少表现二硫酸盐测序 (RRBS) 来识别差异甲基化区域 (DMR).

主要成果:

暴露于CNM改变了DNA甲基化模式,未消化的样本倾向于超甲基化,而消化的样本倾向于低甲基化.
在对未消化的和已消化的CNMs的反应中,发现了显著的DMR和差异甲基化基因.
路径分析表明,CNMs影响了信号传导,甘氨酸甘氨酸代谢,糖化,DNA修复,葡萄糖代谢和细胞骨/细胞外矩阵动态.

结论:

CNM对肠道细胞产生生物效应,特别是影响DNA甲基化.
表观遗传学分析对于阐明CNM毒性的机制至关重要.
这些发现强调了在口服纳米材料的毒理学评估中考虑消化的重要性.

关键词:

通过DNA甲基化.基因本体学是基因的本体学.在体外消化过程中消化.纳米纤维素的使用方法纳米材料是一种纳米材料.路径分析路径分析

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