提升生物膜去除:评估电解方法,以消毒牙植入物在体外
Ana B Castro1, Jill Hadisurya1, Mrinal Gaurav Srivastava2
1Department of Oral Health Sciences, Periodontology and Oral Microbiology, KU Leuven and University Hospitals Leuven, Leuven, Belgium.
Journal of periodontal research
|March 13, 2026
概括
研究人员在种类繁多的口腔生物膜上测试了消毒方法,这些生物膜在植入物上生长. 该研究使用先进的成像和量化技术来评估治疗的有效性,以确定干预后生物膜的持久性.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 牙科植入物学 牙科植入物学
背景情况:
- 口腔生物膜对牙科植入物成功构成重大挑战.
- 有效的净化策略对于管理植入周感染至关重要.
- 植入物在修复性牙科中被广泛使用.
研究的目的:
- 评估不同污染消除策略对多种口服生物膜的有效性.
- 评估治疗后植入物上口服生物膜的持久性.
- 为了比较评估生物膜去除的定量和定性方法.
主要方法:
- 在受控生物反应器中的植入物上建立多种口腔生物膜.
- 在已建立的生物膜上应用各种污染清除协议.
- 定量实时PCR (v-qPCR) 用于生物膜量化.
- 扫描电子显微镜 (SEM) 和共聚焦激光扫描显微镜 (CLSM) 用于形态和空间分析.
主要成果:
- 生物膜的持久性在不同的净化策略中有显著差异.
- v-qPCR提供了剩余生物膜生物质的定量数据.
- SEM和CLSM可视化了残留生物膜的结构完整性和分布.
- 某些清除污染方法在减少生物膜负载方面表现出更高的有效性.
结论:
- 选择污染清除策略对从植入物中移除口腔生物膜产生了重大影响.
- 量化和成像技术的结合对于全面评估治疗疗效至关重要.
- 需要进一步的研究来优化消毒协议,以改善植入式牙科的临床结果.
相关概念视频
Methods of Sterilization II: Chemical Methods
10.3K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
10.3K
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
1.4K
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
1.4K
Methods of Sterilization I: Physical Methods
25.0K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
25.0K


