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标识和特征 乳酸酶贝西勒斯帕拉凯西 HKWS110 从Prunus yedoensis中分离出来
Narangerel Mijid1, Batchimeg Namshir2, Gil-Ha Kim2
1School of Animal Science and Biotechnology, Mongolian University of Life Science, Ulaanbaatar 17024, Mongolia.
Food science of animal resources
|March 13, 2026
概括
一种新的乳酸细菌Lacticaseibacillus paracasei从Prunus yedoensis中分离出来. 这种益生菌菌株表现出极好的耐酸性,抗氧化和抗菌性质,使其适合食品发酵.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- 乳酸细菌 (LAB) 在食品发酵中至关重要,并具有益生菌潜力.
- 研究LAB的新来源,如Prunus yedoensis,可以产生具有有益性质的菌株.
研究的目的:
- 为了从Prunus yedoensis中分离和识别LAB.
- 评估益生菌特征,包括安全性,耐酸性,抗氧化和抗菌活动的孤立菌株.
- 在不同的条件下评估抗菌活性的稳定性.
主要方法:
- 使用16S rRNA测序来隔离和识别LAB.
- 评估耐酸性和血溶性活性,以评估安全性.
- 使用DPPH激素清除试验测量抗氧化剂活性.
- 使用纸盘扩散和96井板方法对沙门氏菌和大肠杆菌的抗菌活性的评估.
- 测试抗菌活性对热量和pH值变化的稳定性.
主要成果:
- 一个被称为Lacticaseibacillus paracasei (99.9%同源) 的菌株被分离出来.
- 该菌株在酸性条件下存活率为72.63%,并且非血溶性,表明安全性.
- 没有细胞的超自然体显示出显著的抗氧化活性 (70.40%的DPPH清除) 和对沙门氏菌和大肠杆菌的抗菌作用.
- 抗菌活性是热稳定的,但在中性pH下会消失,这表明有机酸是主要的抑制化合物.
结论:
- 从Prunus yedoensis中分离出来的Lacticaseibacillus paracasei具有显著的益生菌潜力.
- 该菌株的安全性,耐酸性和强大的抗菌和抗氧化特性支持其在食品发酵和益生菌产品中的应用.
关键词:
乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶 乳糖酶普鲁努斯 (Prunus yedoensis) 是一个植物.它具有抗菌活性,具有抗菌活性.乳酸细菌是一种乳酸细菌.益生菌是一种益生菌.更多相关视频
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