相关实验视频

Updated: Mar 14, 2026

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Published on: March 19, 2021

在牛胚胎发育过程中mTOR通路活性的空间动态

Francieli P Berling¹, Viviane B G Bacaro¹, Ricardo I de Paschoal¹

¹Laboratories of In vitro Fertilization, Cloning and Animal Transgenesis, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction, University of Sao Paulo, Sao Paulo, Brazil.

Frontiers in cell and developmental biology

|March 13, 2026

概括

对于细胞生长至关重要的mTOR通路活性在早期胚胎发育期间,在牛皮细胞中升高. 这表明了mTOR的存在.

科学领域:

细胞和发育生物学
分子信号通路分子信号通路
胚胎学胚胎学

背景情况:

拉巴胺素 (mTOR) 途径的机械性标是细胞生长,增殖,新陈代谢和生存的中央调节者.
营养素和生长因子的可用性显著影响mTOR活动,影响胚胎发育.
在小鼠模型中,mTOR信号传递与皮 (TE) 形成有关,这是早期胚胎中的第一个分化事件.

研究的目的:

为了研究一种假设,即mTOR通路活性在分化为TE的细胞和早期牛胚胎的已建立的TE细胞中增加.
描述牛在植入前发育过程中mTOR活动的时间和空间动态.

主要方法:

化S6 (pS6) 的免疫光检测,这是mTOR激活的下游标志物.
焦显微镜用于牛胚胎pS6局部化的高分辨率成像.
分析pS6表达在Morula,早期胚胎细胞和晚期胚胎细胞阶段.

主要成果:

-S6 (pS6) 信号,表明mTOR活动,被检测到在牛的外细胞 morulae.
在早期牛胚胎囊的 trofhectoderm (TE) 中观察到较高的ps6活性.
晚期牛胚囊细胞的TE中pS6信号减少,表明mTOR活动的短暂模式.

结论:

在早期牛胚胎发育过程中,mTOR通路的激活特异地局限于皮细胞.
观察到的模式表明mTOR信号在牛中TE分化和/或功能中起着至关重要的作用.
需要进一步的研究来阐明mTOR在牛TE发育中的确切功能.

关键词:

不同化的差异化差异化.内细胞质量细胞质量.核 mTOROR 的使用情况.素-S6 的使用.皮肤上的托菲克托多德.

更多相关视频

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Published on: June 26, 2018

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...