探索不同Listeria monocytogenes菌株在寒冷压力下细胞外代谢物的调节
Hyun-Jun Kim1, Hye-Jin Kim1, Cheorun Jo1,2
1Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Food science of animal resources
|March 13, 2026
概括
经过冷应激的Listeria monocytogenes会改变其细胞外代谢物,增强能量和谷氨通路. 特定的代谢物度可以识别其存在,有助于食品安全.
科学领域:
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
- 食品安全 食品安全
背景情况:
- 李斯特菌 (Listeria monocytogenes) 是一种通过食物传播的病原体,需要了解其生存机制.
- 寒冷压力是食品保存的常见挑战,影响微生物新陈代谢.
- 细胞外代谢物概况可以揭示微生物适应策略.
研究的目的:
- 为了研究冷压如何调节不同Listeria monocytogenes菌株的细胞外代谢物.
- 为了确定在寒冷条件下表明Listeria monocytogenes存在的特定代谢物.
- 为了探索Listeria monocytogenes在长时间暴露在寒冷中代谢途径的适应.
主要方法:
- 培养3种Listeria monocytogenes菌株 (L5,L6,L9) 在Mueller Hinton中在8°C下长达22天.
- 在五个不同的生长阶段 (初始,滞后,日志,早期和,和) 提取细胞外代谢物.
- 分析代谢物度以区分菌株和识别代谢途径变化.
主要成果:
- 在寒冷压力下,Listeria monocytogenes的生长模式在L5/L6和L9菌株之间有所不同.
- 细胞外代谢物变化依赖于生长阶段和细菌菌株.
- 三糖,单氨酸,氨酸和氨酸度区分了Listeria monocytogenes的存在.
- 能量代谢和与谷氨相关的代谢途径在滞后和日志阶段得到增强.
结论:
- 细胞外代谢物表达提供了对Listeria monocytogenes寒冷压力适应的见解.
- 开发了一种基于代谢物概况的新方法,用于在寒冷压力下识别Listeria monocytogenes.
- 这些发现对改善食品行业对Listeria monocytogenes的安全措施有潜在的应用.
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