卵泡液中的活性氧物种作为卵细胞发育能力的潜在生物标志物
Sara Borjian-Boroujeni1, Naser Shams-Esfandabadi1,2, Abolfazl Shirazi3
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Journal of reproduction & infertility
|March 13, 2026
概括
在水牛的卵泡液中,总氧化剂状态 (TOS) 的高水平表明卵细胞发育能力不佳. 较低的TOS与更好的胚胎质量有关,这表明TOS是辅助生殖技术成功的生物标志物.
科学领域:
- 生殖生物学 生殖生物学
- 氧化压力研究研究 氧化压力研究
- 动物生物技术动物生物技术
背景情况:
- 活性氧物种 (ROS) 对卵细胞和胚胎发育至关重要,但过度水平会导致氧化应激,损害线粒体功能和DNA完整性.
- 牛毛囊液 (FF) 中氧化状态对生殖结果的影响尚不清楚.
研究的目的:
- 调查FF水牛的总氧化状态 (TOS) 与卵细胞的发育能力之间的关系.
- 评估TOS作为预测水牛卵细胞和胚胎质量的潜在生物标志物.
主要方法:
- 从62只水牛中收集了毛囊液和积卵细胞复合物 (COC).
- 卵子经历了体外成熟和受精;动物按胚胎细胞产量分组 (G1: 0,G2: ≥2,G3: >3).
- 总氧化剂状态 (TOS) 通过光谱测量进行测量,并使用ROC和Spearman相关性进行分析.
主要成果:
- 与没有产生的水牛 (G1) 相比,产生更多胚芽细胞的水牛 (G2) 的FF TOS水平明显较低 (p=0.004).
- 1.12μmol H2O2 Eq/L的TOS值被确定为卵细胞发育能力的预测指标 (AUC=0.851).
- 升高的TOS与高质量的胚胎囊的比例相反相关 (r=-0.553,p=0.021).
结论:
- 氧化应激增加,FF中的高TOS表明,对水牛的卵细胞发育能力和胚胎质量产生负面影响.
- 在FF中的TOS显示了作为卵细胞质量预测生物标志物的潜力.
- 测量TOS可能有助于优化对水牛辅助生殖技术 (ART) 的抗氧化剂策略.
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