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SPIN:用于可扩展和灵敏单细胞蛋白质学的喷墨驱动纳米孔工作流.

Eric Cheng¹, Shuxin Chi², Huan Zhong²

¹Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.

Analytical chemistry

|March 13, 2026

概括

我们开发了一个自动化的单细胞打印机和纳米孔芯片,用于单细胞蛋白质组学. 该系统提高了蛋白质的恢复和可重复性,使得可扩展的单细胞蛋白质组分析成为可能.

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科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
细胞生物学细胞生物学

背景情况:

单细胞蛋白质组学对于理解细胞异质性至关重要.
目前的样本处理方法面临的挑战是有限的输入材料和缺乏蛋白质放大.
有效和可重复的样本制备对于推进单细胞蛋白质组学至关重要.

研究的目的:

提出使用集成喷墨打印机和纳米孔芯片的单细胞蛋白质组学的新方案.
克服样品处理的局限性,包括材料损失,低吞吐量和糟糕的可重复性.
为了实现可靠和可扩展的单细胞蛋白质组分析.

主要方法:

开发一种图像引导,机器学习驱动的喷墨单细胞打印机.
与露点控制的纳米井芯片集成,用于精确的细胞分配和环境调节.
使用高热导电性基板,以提高热稳定性.
通过可密封的纳米升井和减少表面接触来最大限度地减少样品损失.

主要成果:

实现了以高于1Hz的速度分发单细胞.
与商业配剂器相比,显示出明显更高的蛋白质和回收.
观察到均的深层蛋白质覆盖,没有偏向于高丰度蛋白质.
在A549细胞和星球细胞中证实了克雷布斯循环酶的近100%蛋白质完整性,表明其具有很高的可重复性.

结论:

提出的平台有效地解决了单细胞蛋白质组学样本处理中的核心瓶.
该系统可实现可靠,可扩展和高度可复制的单细胞蛋白质组分析.
这一进步通过增强的蛋白质组分辨率,促进了对细胞异质性的更深入的洞察.