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城市污水抗体部分反映了临床抗体

Alix Vincent Thorn¹, Christian Brinch¹, Frank M Aarestrup¹

¹Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.

|March 13, 2026

概括

对抗微生物耐药性基因 (ARG) 的污水监测显示了与临床数据的一些相关性,但错过了许多广泛的ARG. 这凸显了仅仅使用废水进行全面的全球抗菌耐药性跟踪的局限性.

科学领域:

环境微生物学环境微生物学
公共卫生基因组学
传染病监测传染病监测传染病监测传染病监测

背景情况:

抗菌素耐药性 (AMR) 是一个关键的全球卫生挑战,需要强有力的监测.
抗微生物耐药性基因 (ARG) 是AMR的关键指标.
污水监测为追踪ARG提供了传统临床隔离分析的潜在替代方案.

研究的目的:

在全球范围内比较检测废水元基因组中的ARG及其变体与临床细菌分离物的检测.
评估基于污水和基于临床隔离物的ARG监测之间的一致性和差异.
评估污水监测对于捕获临床相关ARG的全谱的实用性.

主要方法:

对来自33个国家的2,989个临床细菌分离体和468个污水元基因组的ARG数据进行比较分析.
利用读取映射来识别污水元基因组中的ARG.
使用斯皮尔曼相关性来评估污水中的ARG丰度与临床隔离物检测率之间的关系.

主要成果:

在两种数据集中都观察到显著的区域模式,但许多ARG变体仅在污水或临床隔离物中被检测到.
只有69%的临床分离的ARG集群在污水转基因组中被检测到.
在污水中没有检测到一些高度流行的临床ARG,并且相关性因ARG和细菌物种而异.

结论:

污水ARG监测与临床AMR格局相关,但不包括目前测序深度临床意义上的ARG的完整范围.
对于某些细菌物种和感染类型中普遍存在的特定ARG,基于废水的监测可能更有效.
考虑这些局限性在建立针对AMR的污水监测系统时至关重要.

关键词:

抗生素耐药性抗生素耐药性临床方法临床方法进行比较性研究.计算生物学是计算生物学.遗传学遗传学遗传学是一个基因组学就是基因组学.转基因组学是指转基因组学.微生物生态学微生物生态学监督研究监督研究监督研究废水是废水,废水就是废水.

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